两种处理方法对精子顶体完整性和DNA损伤的影响

Impact of two sperm selection techniques on sperm acrosome integrity and DNA fragmentation

目的研究上游法和密度梯度离心法处理对精液常规参数、顶体完整性及DNA损伤的影响。方法 90例男性精液标本用上游法和密度梯度离心法处理,检测处理前后精液常规参数、精子顶体完整率及精子DNA碎片指数。结果两种方法处理后,精子浓度明显下降(P<0.01),精子活率、活力明显提高(P<0.01);正常形态精子比例和顶体完整率显著提高(P<0.05),DNA碎片指数显著降低(P<0.01),两种处理方法比较差异无统计学意义。结论两种处理方法均可以使顶体完整精子比例较处理前明显提高,DNA损伤精子比例较处理前明显降低,但两法之间无统计学差异。两种处理方法均可以不同程度优化精液质量,实验室可根据精液情况选择处理方法。

Objective To investigate the effect of two methods（swim-up and pure sperm gradient centrifugation）on sperm routine test,acrosome integrity and DNA fragmentation.Methods Semen sample of 90 patients were treated with swim-up and pure sperm gradient centrifugation,and then routine parameters,sperm acrosome integrity and sperm DNA fragment before and after treatment were analyzed.Results The sperm concentration decreased markedly after treated by the two methods and the survival rate and sperm mobility increased obviously（P0.01）.The rate of normal sperm morphology and acrosome integrity increased significantly（P0.05）.The index of sperm DNA fragment decreased markedly（P0.01）.No difference in treatment were noticed between the two methods.Conclusions The acrosome integrity was increased,sperm DNA fragment decreased after treatment with the two methods.The sperm quality can be optimized by using the two methods.