红皮沙拐枣果实红色素提取工艺优化及其抑菌活性的研究

Extraction and antibacterial activity of red pigment from Polygonum fruit

对红皮沙拐枣果实红色素的提取条件及抑菌作用进行了系统研究,结果表明：沙拐枣色素的最大吸收波长为λmax=325nm,最适宜的提取剂为乙醇,最佳的提取条件为：提取剂浓度为85%、提取剂pH为3、提取温度为50℃、提取时间为3h,固液配比1：15。该色素对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和变形杆菌的生长有较强的抑制活性,最低抑菌浓度（MIC）分别为2、2、6和4mg/mL;该色素抑菌pH范围为3~6,高温对其抑菌作用具有一定的影响;而低浓度的还原剂对其抑菌作用影响不明显。

Extraction and antibacterial activity of red pigment from Polygonum fruit.The pigment has the maximum absorption wavelength λmax=325 nm.The satisfactory solvent is alcohol.The optimum conditions were： concentration of solvent 85%,pH=3 and temperature of extraction 50 ℃.The red pigment had stronger antibacterial activity to Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Bacterium vulgarus.The minimum inhibiting contents （MIC） were 2,2,6,4 mg/mL respectively.The pH range of inhibiting bacteria as 3～6,the low concentration reducing agent basic has not affected to the pigment’s antibacterial function; Moreover,the heat stability was not good.