摘要
为了简化烟草样品的前处理步骤,采用甲醇直接萃取烟草样品得到甲醇萃取液,优化超高效液相色谱的测试条件,测定了甲醇萃取液中的茄尼醇含量。结果表明:①最佳萃取条件为:萃取溶剂为甲醇。萃取温度25℃,萃取时间20min;②茄尼醇工作曲线的浓度范围为10~200mg/L时,质量浓度与峰面积具有良好的线性关系,相关系数为0.9997;③方法的检出限为0.054mg/L,相对标准偏差为2.1%,平均回收率为105.5%。该方法可以快速、简便、准确地测试烟草中游离茄尼醇的含量。
In order to simplify the pretreatment procedures, tobacco sample was directly extracted by methanol and the UPLC-UV conditions were optimized, then the solanesol content in the obtained extract was determined. The results showed that: 1) The optimal conditions of extraction were: methanol as solvent and extracting for 20 minutes at 25 ℃. 2) The concentration of working curve of this method ranged from 10 to 200 mg/L. Good linear relationship existed between mass concentrations and peak areas with the correlation coefficient of 0.9997; 3) The detection limit of this method was 0.054 mg/L with the relative standard deviation of 2.1% and the average recoveries of 105.5%. This method is fast, simple, accurate and suitable for the determination of solanesol content in tobacco.
出处
《烟草科技》
EI
CAS
北大核心
2013年第5期61-63,71,共4页
Tobacco Science & Technology
基金
中国烟草总公司科技重大专项项目"卷烟主流烟气中苯并芘的形成机理研究"(110201001001)
云南中烟工业公司科技开发计划项目(2012JC02)