α-干扰素对人肺腺癌A_(549)细胞周期和P53蛋白表达的影响

Influences of α-interferon on the cell cycle and P53 expression in the human lung adenocarcinoma cell line(A_(549))

目的：探讨α－干扰素（ＩＦＮ－α）对人肺腺癌细胞株Ａ５４９细胞周期和Ｐ５３蛋白表达的影响。方法：用２种不 同质量浓度ＩＦＮ－α（２５μｇ／Ｌ，１００ μｇ／Ｌ）处理人肺腺癌Ａ５４９细胞，并设空白为对照。用流式细胞仪测定ＩＦＮ－α对Ａ５４９细 胞细胞周期的影响，用免疫荧光标记法测定Ｐ５３蛋白的表达。结果：ＩＦＮ－α作用后的Ａ５４９细胞，细胞周期明显改变，Ｓ期细 胞数显著降低，且Ｐ５３蛋白表达明显减少。结论： ＩＦＮ－α能阻止Ａ５４９细胞细胞周期的进程，抑制Ｐ５３蛋白的表达。

Aim: To investigate the influences of α-interferon(IFN-α)on the cell cycle and P53 expression in the human lung adenocarinoma cell line (A549). Methods: The human lung adenocarcinoma cell line(A549)was treated with IFN-α(25 μg/L, 100μg/L), P53 expression and cell cycle was determined with immunohistochemisty and flow cytometry(FCM).Results: P53 expression was significantly decreased in the A549 cell line treated with IFN-α.The cell cycle in the A549 cells was treated with IFN- -α was changed markedly, and the cells in S phase were decreased dramatically.Conclusion: IFN-α could arrest the cell cycle of A549 cells and inhibite the P53 expression.