伊宁市19例HIV-1感染者gag基因序列分型研究

Study on Subtypes of Gag Gene of HIV-Infected 19 Cases in Ili City

目的:通过扩增伊宁市维吾尔族HIV-1感染者gag基因片段,确定该民族HIV-1感染者流行株的基因亚型。方法:抽提20例新疆伊宁市HIV-1感染者的血浆标本的RNA,用巢式PCR扩增gag基因片段,将所得到的序列与国际标准株进行比较,确定被检标本的亚型。结果:在20例HIV感染者的血浆标本中,共成功扩增19例gag基因片段,扩增率为95%。经BLAST初步判断和构建进化树并与国际标准株比对确认,19例感染者感染的HIV毒株序列中,发现18例为CRF07_BC亚型,1例为C亚型。19例毒株之间的平均基因距离为0.043±0.003,与3例CRF07_BC亚型国际参考株之间的平均基因距离在0.034±0.004-0.039±0.005之间。结论:新疆伊宁市HIV-1感染者以CRF07_BC亚型为主要流行株。19例序列相互之间以及与3株不同来源的国际参考毒株之间表现出高度的同源性。

Objective：To explore the prevalent subtype of HIV-1 infected patients in Urumqi Uyghur, gag gene was amplified. Methods To extract HIV-1 RNA from HIV-1 positive plasma from 68 HIV-1 infected patients in Urumqi, then HIV-1 gag gene were amplified by nest-PCR, and was further subject to the DNA sequence and subtype analysis. Results 35 study subjects could successful be performed gag gene PCR. The rate was 51.47%. One HIV-1 subtypes, CRF07_BC（35 strains）,is identified. Average gene distance of the 35 sequence was 0.043 ± 0.003.The average gene distance to the 3 international standard strains were between 0.034 ± 0.004 and 0.039 ± 0.005. Conclusions The prevalent subtype of HIV-1 infected patients in Ummqi was CRFO7_BC. All the 35 sequences and 3 international standard strains were highly homogenous.