摘要
目的采用高效液相色谱法测定红宝散中欧前胡素的含量。方法采用HPLC法,Apollo-C18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇-水(55∶45),流速为1.0mL·min-1,紫外检测波长为300nm,柱温为室温。结果欧前胡素进样量在0.0300～0.2000ug线性关系良好,r=0.9999,平均回收率99.68%,RSD=0.35%。结论本方法准确、简便、快速,可作为红宝散中欧前胡素的含量测定。
Objeetive To establish a method for the determination of Imperatorin in Hong Bao powder. Methods HPLC method was used to deter- mine the content. The column was Apollo -C18（250 minX4. 6 ram,5μm） ,and the mobile phase：methanol-water （55 ： 45）. The flow rate was 1.0 mL · rain-1 and the detective wavelength was 300nm. Results The calibration curve of Imperatorin was linear in the range of 0. 0300 0. 2000 μg（ r = 0. 9999）, the average recovery was 99.68 % , RSD = 0. 35 %. Conclusion The method is accurate with good reproducibility and can be used for the content determination of Imperatorin in Hong Bao powder.
出处
《中国中医药现代远程教育》
2013年第8期149-150,共2页
Chinese Medicine Modern Distance Education of China
