高糖条件下血管内皮细胞增殖与凋亡的研究

Study on proliferation and apoptosis in cultured vascular endothelial cells with high glucose concentration

目的 观察高糖对血管内皮细胞增殖、凋亡的影响。 方法 用高糖 (10、2 0、30、40、5 0mmol/L)作用培养的人脐静脉血管内皮细胞 (humanumbilicalveinendothelialcells ,HUVEC) ,第 2、4、6、8、10、12、14d ,用氚 标记脱氧胸苷 (tritium labelledthymidinedeoxyribose ,3H TdR)掺入法测定每分钟计数 (countsperminute ,cpm) ,流式细胞术检测细胞的凋亡指数、增殖指数及细胞周期变化。 结果 高糖作用下 ,HUVECcpm值和增殖指数低于对照组 (P <0 .0 5 ) ,凋亡指数高于对照组 (P <0 .0 5 ) ,随作用浓度增加、时间延长更为明显。各高糖组G1期细胞百分率升高 ,S期百分率降低 (P <0 .0 5 )。 结论 高糖使培养的HUVEC凋亡加速 ,同时抑制了细胞增殖。细胞可能被阻滞在G1/S转变期。

Objective To investigate the effect on proliferation and apoptosis of vascular endothelial cells exposed to high glucose. Methods The cultured human umbilical vein endothelial cells (HUVEC) were treated with high glucose at concentrations of 10,20,30,40,50 mmol/L for 2,4,6,8,10,12,14 days,cpm value was studied by using tritium-labelled thymidine deoxyribose( 3H-TdR)incorporation assay.Flow cytometry was used to detect apoptosis index,proliferation index and cell cycle. Results Treated with high glucose,the proliferation index and cpm were significantly reduced in a dose and time dependent manner than the control groups(P<0.05).The apoptosis index of HUVEC were higher compared with the control groups(P<0.05).The percent of the cultured cells in G1 phase in all high glucose groups were increased, the percent in S phase were largely decreased(P<0.05). Conclusion Exposed to high glucose,the apoptosis of HUVEC was accelerated and the suppressive effect of high glucose on the proliferation of HUVEC was observed.Endothelial cells were possibly arrested in G1/S checkpoint.