利用重组痘苗病毒表达人 CD2 0基因

Expression of human CD20 gene in recombinant vaccinia virus

目的获得重组人 CD2 0分子并研究编码人 CD2 0的基因在痘苗病毒中的表达。方法从 p GEM- T- EASY/ CD2 0载体上酶切下编码人 CD2 0分子的 c DNA,亚克隆到 p JSA1175载体上 ,重组质粒与野生痘苗病毒共转染 TK- 143细胞。结果APAAP检测到重组病毒感染的细胞表面有 CD2 0分子表达 ,富集后病毒滴度约为 1× 10 9pfu/ ml。结论人 CD2 0基因在痘苗病毒中表达 。

ObjectiveTo obtain human recombinant CD20 molecule and study the expression of human CD20 in vaccinia virus. MethodsThe full length gene of CD20 was inserted at the SmaⅠ site of pJSA1175 expression vector under the control of 7.5 K promoter. The recombinant plasmid were used to construct recombinant vaccinia virus. ResultsCD20 molecule was expresssed in the membrane of the infected cells. Tilter of the recombinant vaccinia virus is 1×10 9 pfu/ml. Conclusion This experiment provides a new approach to study the function of CD20 which will also lay a foundation of preparation for the mAb against CD20