阿米巴肝脓肿患者血清细胞因子水平与免疫防卫能力的研究

Study on level of cytokines in sera and immune defense ability of patients with amoebic liver abscess

目的了解阿米巴肝脓肿患者机体的免疫防卫能力。方法 EL ISA、比色法及流式细胞仪等检测了 40例阿米巴肝脓肿患者血清细胞因子 TNF- α、IL- 6、IL- 8、s IL- 2 R、弓形虫感染率、NO、血液 CD4/ CD8T细胞、红细胞 C3b受体花环率(RBC- C3b RR)及免疫复合物花环率 (RBC- ICR)。结果 TNFα、IL- 6、IL- 8、s IL- 2 R、NO、RBC- ICR显著高于正常对照组 ;RBC-C3b RR低于正常对照组 ;CD4增高 ,CD8降低 ,CD4/ CD8比值下降 ;弓形虫感染率与正常组无差异。结论阿米巴肝脓肿患者非特异性细胞免疫受抑制、特异性细胞免疫增强 。

ObjectiveTo investigate the immune defense ability of patients with amoebic liver abscess. Methods ELISA、colorimetry and flow cytometry were used to detect TNF α,IL 6,IL 8,sIL 2R,toxoplasma infection rate(ToR),NO and CD4/CD8 T cell in 40 patients with amoebic liver abscess.The red cell function was measured at the meantime by detec ting RBC C3bRR and RBC ICR in membrane of red cell.ResultsThe level of TNF α,IL 6,IL 8,sIL 2R,NO and RBC ICR in patients was higher than those found in the control group.RBC C3bRR was lower than that in the control group.The number of CD4 T cell in group of patients decreased,while that of CD8 T cell increased.No significant difference existed between ToR found in patients and control group.ConclusionThe non specific cell mediated immunity was suppressed in patients with amoebic liver abscess while specific cell mediated immunity enhanced.The immune defense ability of the patients was normal. [