碳铂壳聚糖微球对胶质瘤细胞作用的形态学研究

Morphological characterization of chitoson polymer microsphere with carboplatin treat cultured C 6 glioma cell in vitro

目的 :在体外培养C6胶质瘤细胞时加入自制的碳铂壳聚糖缓释微球 ,通过形态学观察确定肿瘤细胞与药物的互动关系。方法 :利用相差显微镜进行动态观察 ,并辅以扫描电镜进行形态学的观察。结果 :通过观察我们发现药物微球球面光整 ,表面无结晶 ,释药 2周后球体坍塌率小于 2 % ;而在扫描电镜下微球的超微结构观察显示微球的外壳首先脱落出现“脱皮现象” ,然后药物晶体通过溶渗作用在微球表面形成众多的微孔 ,而在此后的过程中药物球体大致保持完整 ,球体坍塌率小于 2 %。Giemsa染色及扫描电镜均可观察到明显的肿瘤细胞的凋亡现象 ,而且具有明显的浓度依赖性和距离依赖性 ,流式细胞仪计数以确认凋亡细胞群的存在。结论 :我们的碳铂壳聚糖微球的药物控释机制是药物晶体从载体结构中通过溶渗作用逐渐释放的 ,药物微球的刚性极佳。药物微球具有明显的抑制肿瘤生长的作用 。

Objective:We use the chitoson polymer microsphere carried with carboplatin treat cultured C 6 glioma cell in vitro. Through the morphological observation, we went to demonstrate the relationship between drug and C 6 glioma cell. Methods:We use reversed microscope and scanning electron microscope to observe chitoson polymer microsphere carried with carboplatin treat cultured C 6 glioma cell in vitro. Results:We find drug microspheres have a good appearance and very stable structure. The ruin rate of drug microspheres is lower than 2% after two weeks. Under the scanning electronic microscope we also observed that the microsphere with carboplatin take off its coat first and then appears many micro holes on their surface. Apoptosis were demonstrated by observation of scanning electronic microscope, Giemsa staining and FCM. Conclusion:Remarkably, the drugs released from microspheres through these holes, but the skeleton of polymer microspheres keeps entirety. Drug microsphere can inhibit cultured C 6 glioma cells in vitro.