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PSP57不是增生和癌变前列腺组织PSP94 mRNA可变剪切特有的产物

PSP57: A non-specific product of PSP94 mRNA variable splicing in prostatic tissues from patients with prostate carcinoma and benign prostatic hyperplasia
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摘要 目的 :比较人正常、增生和癌变前列腺组织中PSP94和PSP5 7mRNA的表达情况。方法 :提取事故死亡的正常成年人前列腺组织及手术得到的增生和前列腺癌组织总RNA ,进行RT PCR ,其产物分别以PSP94和PSP5 7共有的外显子及PSP94外显子Ⅲ作探针进行Southernblotting分析 ;RT PCR产物经克隆后进行序列测定。结果 :三种前列腺组织中均有PSP94和PSP5 7mRNA的表达 ;人正常前列腺组织中的PSP94及PSP5 7的基因序列与增生前列腺组织和癌变前列腺组织中的完全一致。结论 :PSP5 Objective:Analysize and compare the expression of human PSP94 and PSP57 mRNA in prostatic tissues from normal adults, patients with prostate carcinoma and benign prostatic hyperplasia (BPH).Methods:Extract total RNA in prostatic tissues taken by operation from normal adults died of accident, patients with prostate carcionma and BPH, then do reverse transcriptase polymerase chain reaction (RT PCR). RT PCR products are analysized by sequence determination and Southern blotting using specific exon Ⅱ of PSP94 and PSP57 and exon Ⅲ of PSP94 as probes.Results:The PSP94 and PSP57 mRNA are both found in the above three kinds of prostatic tissues. The cDNA sequences of PSP94 and PSP57 in normal prostatic tissue are consistent with that in carcinoma and hyperplasia tissues. Conclusion:PSP57 is a non specific product of PSP94 mRNA in prostatic tissues from patients with prostate carcinoma and benign prostatic hyperplasia.
出处 《军医进修学院学报》 CAS 2000年第3期218-220,共3页 Academic Journal of Pla Postgraduate Medical School
关键词 PSP57 PSP94mRNA 前列腺增生 前列腺癌变 prostate prostate secretory protein PSP57 PSP94
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参考文献7

  • 1Xuan JW,Chin JL,Guo YZ,et al.Alternative splicing of PSP94 (prostatic secretory protein of 94 amino acids) mRNA in prostate tissue[].Oncegene.1995
  • 2Nolet S,Mbikay M,Chretien M.Prostatic secretory protein PSP94: gene organization and promoter sequence in Rhesus monkey and human[].Biochemistry and Biophysics.1991
  • 3Mundle SD,Sheth NA.Suppression of DNA synthesis and induction of apoptosis in rat prostate by human seminal plasma inhibin(HSPⅠ)[].Cell Biology International.1993
  • 4Linard CG,Mbikay M,Benjannet S,et al.Correct processing and secretion of a human prostatic secretory protein (PSP94) in Escherichia coli[].Gene.1988
  • 5Liu AY,Bradner RC,Vessella RL.Decreased expression of prostatic secretory protein PSP94 in prostate cancer[].Cancer Letters.1993
  • 6Green CB,Liu WY,Kwok SCM.Cloning and nucleotide sequence analysis of the human β-microseminoprotein gene[].Biochemical and Biophysical Research Communications.1990
  • 7Garde SV,Sheth AR,Porter AT,et al.A comparative study on expression of prostatic inhibin peptide , prostate acid phosphatase and prostate specific antigen in androgen independent human and rat prostate carcinoma cell lines[].Cancer Letters.1993
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