细胞周期相关调控蛋白在胰腺癌中的表达及其意义

THE EXPRESSION OF CELL CYCLE REGULATED PROTEINS IN PANCREATIC ADENOCARCINOMA AND ITS SIGNIFICANCE

目的 了解细胞周期相关调控蛋白在胰腺癌中的表达及其对胰腺癌细胞增殖的影响。方法 应用高压锅抗原修复免疫组化ABC法 ,检测 47例胰腺癌标本的细胞周期相关调控蛋白 (p5 3、p16、p2 1及Rb蛋白 )的表达 ,并用一种新的ki s5抗体检测胰腺癌细胞ki 6 7增殖指数。结果 p5 3、p16、p2 1及Rb蛋白阳性表达均位于细胞核 ,胰腺癌中 p5 3、p16、p2 1和Rb蛋白表达阳性率分别为 5 5 % (2 6 /47)、5 3 % (2 5 /47)、6 4% (35 /47)和 98% (46 /47) ;p16、p2 1及Rb蛋白表达与ki 6 7增殖指数呈负相关 ;p5 3蛋白表达与p2 1蛋白表达无关。 结论 大部分胰腺癌的异常增殖可能与 p16、p2 1蛋白阴性表达有关 ,而由Rb蛋白阴性表达导致的胰腺癌细胞的异常增殖少见。p5 3蛋白表达与 p2

HT6”,5”] [WT5”BZ] [WT5”HZ]Objective [WT5”BZ]To know the abnormal expression of the cell cycle regulated proteins in pancreatic adenocarcinoma and their effect on tumor cell growth. [WT5”HZ]Methods [WT5”BZ]The expression of p16, p21, Rb and p53 protein in 47 cases were investigated by immunohistochemistry with wet autoclave pretreatment for antigen retriaval. Furthermore, tumor growth index were assessed by a novel anti ki 67 antibody (ki s5). [WT5”HZ]Results [WT5”BZ]All the expression of p53, p16, p21 and Rb protein were the nuclear stainning. The positive rates of p53, p16, p21 and Rb protein were 55%, 53%, 74% and 98% respectively. There was negative correlation between of p16, p21 or Rb protein expression and ki 67 growth index. No relation of p53 protein stainning and the expression of p21 protein was found. [WT5”HZ]Conclusion [WT5”BZ]In pancreatic adenocarcinoma, the negative expression of p16 protein and p21 protein may play an important role in tumor cell growth, but tumor proliferation caused by abnormality of Rb protein is rare. The expression of p21 protein was not associated with the expression of p53 protein. [HT6,5”] [WT5”HZ]