G市某区管网末梢水中有机提取物对雄性大鼠生殖激素分泌水平的影响

Effects of Organic Pollutants from a Regional Pipeline Water Source in G City on Reproductive Hormone Level of Male Rats

[目的]研究G市管网末梢水中有机提取物对雄性大鼠生殖器官重量及生殖激素分泌水平的影响,探讨其雄性生殖毒性作用。[方法]利用固相萃取法提取G市某区管网末梢水中有机污染物。将40只SD雄性大鼠随机分为溶剂对照组(给予玉米油)、低剂量染毒组(3L/kg.d)、中剂量染毒组(15L/kg.d)、高剂量染毒组(75L/kg.d),每组10只。每天灌胃一次,连续染毒28d后处死动物,采集大鼠血清,采用直接化学发光法检测大鼠血清中促黄体生成素(luteinizing hormone,LH)、睾酮(testosterone,T)及雌二醇(E2)的分泌水平。[结果]各染毒组大鼠双侧睾丸、附睾的重量与对照组比较,差异无统计学意义(P>0.05)。随管网末梢水中有机提取物染毒剂量的增加,大鼠血清中LH呈下降趋势,但与溶剂对照组比较,差异无统计学意义(P>0.05)。血清中E2水平随染毒剂量增加明显升高,与对照组及低剂量组比较,中、高剂量染毒组的E2均升高(P<0.05,P<0.01)。随染毒剂量的增加,T分泌水平呈先升高后降低的趋势,但仅高剂量组的降低具统计学意义(P<0.05)。[结论]G市某区管网末梢水有机提取物中、高剂量暴露,可损伤雄性大鼠睾丸间质细胞,抑制睾酮分泌,并诱导E2合成增加,破坏生殖内分泌激素的相对平衡,导致生殖功能受损。

[ Objective ] To analyze the toxic effects of organic pollutants extracted from pipeline water in G City on the reproductive organ weights and hormone levels of male rats. [ Methods ] Organic pollutants in pipeline water samples from a district of G City were extracted by solid phase extraction method. Forty SD adult male rats were divided into solvent control group （administered with corn oil）, low dose group （administered with organic pollutants extraction, OPE, 3 L/kg body weight per day）, moderate dose group （OPE, 15 L/kg body weight per day）, and high dose group （OPE, 75 L/kg body weight per day）. OPE were administered to the rats once a day, and continuously for 28 days. The rats were sacrificed after OPE administration ended and serum samples were collected for luteinizing hormone （LH）, testosterone （T）, and estradiol （E2） measurement by chemiluminescence. [ Results ] Compared with the control group, no obvious changes were observed in the weights of testis and epididymis in the 3 exposed groups （P 〉 0.05）. With the increase of OPE dosage, the level of rats, serum LH decreased, but compared with the solvent control group, no statistical significance existed （P 〉 0.05）. The level of E2 obviously increased in a dose-dependent manner, especially in the moderate and the high dose groups （P 〈 0.05 or P 〈 0.01）. The tevels of T in the 3 exposed groups were ascending at first and followed by descending along with the increment of OPE dosage, but a significant decline was only observed in the high dose group （P〈0.05）. [ Conclusion ] Exposure to moderate and high doses of OPE extracted from the pipeline water in G city can induce damages to testis leydig cells, destroy the balance of reproductive hormones, and finally result in reproductive dysfunction.