焦炉工人DNA损伤与组蛋白H2A单泛素化水平的关系

Relationship between DNA Damage and Monoubiquitinated Histone H2A in Coke Oven Workers

[目的]探讨焦炉工人DNA损伤与组蛋白H2A单泛素化水平的关系。[方法]采用高效液相色谱法对焦化厂106名焦炉工人(接触组)和73名水处理厂工人(对照组)尿中1-羟基芘(1-hydroxypyrene,1-OHP)浓度进行测定,并采用彗星实验和蛋白质印迹法(Western blot)分别检测DNA损伤和组蛋白H2A单泛素化水平。[结果]接触组Olive尾矩(Olive tail moments,OTM)和尿1-OHP的均值分别为0.46和0.34μmol/molCr,均显著高于对照组(分别为0.37和0.21μmol/molCr,P<0.05);而接触组单泛素化组蛋白H2A相对表达水平的中位数为0.61,与对照组(0.91)相比为降低(P<0.05)。以OTM值为应变量,多元线性回归显示自变量尿1-OHP和单泛素化组蛋白H2A进入回归方程,决定系数R2为0.383。[结论]焦炉工人DNA损伤程度与尿1-OHP含量和单泛素化组蛋白H2A相对表达水平关系密切。

[ Objective ] To examine the relationship between DNA damage and monoubiquitinated histone H2A in coke oven workers. [ Methods ] A total of 106 coke oven workers （exposed group） and 73 water treatment workers （control group） were recruited in this study. The levels of urinary 1-hydroxypyrene （1-OHP） were determined using high performance liquid chromatography. Comet assay and Western blot were used to detect the DNA damage and the monoubiquitylation of histone H2A, respectively. [ Results ] The mean Olive tail moments （OTM） （0.46） and urinary 1-OHP concentrations （0.34 ~mol/mol Cr） in the exposed group were significantly higher than those in the control group （0.37 and 0.21 ~tmol/mol cr, respectively, P〈 0.05）. But the median of expression of monoubiquitinated histone H2A （0.61） in the exposed group were significantly lower than that in the control group （0.91, P 〈 0.05）. By taking OTM as the dependent variable, the results of multiple linear regression analysis showed that urinary 1-OHP and the relative expressions of monoubiquitinated histone H2A fit the equation as independent variables, and the coefficient of determination R2 was 0.383. [ Conclusion ] DNA damage and urinary 1-OHP are closely related with the relative expression of monoubiquitinated histone H2A in the coke oven workers.