hsa-miR-17-92 cluster及其同源体靶基因的生物信息学分析

Target genes of hsa-miR-17-92 cluster predicted by bioinformatics analysis

目的对hsa-miR-17-92duster及其同源体进行系统的生物信息学分析，预测其可能参与的生物学过程，为深入研究其在脂肪细胞分化、肥胖发生等过程中的功能与机制奠定基础。方法1．应用PubMed、Google等信息搜索工具查找hsa-miR-17-92cluster及其同源体的所有研究，综述已有研究进展；2．应用miRBase获取hsa-miR-17-92cluster及其同源体的各成员序列，并分析其序列特征及保守性；3．应用美国国家生物技术信息中心（NCBI）blast、NCBImapviewer、基因组生物信息学（UCSC）Browser工具分析hsa-miR-17-92 cluster及其同源体所在基因组的序列特征；4．应用TargetScan5．1，PicTar及miRanda预测hsa-miR-17-92cluster及其同源体靶基因，取三者预测结果的交集，进一步进行功能注释和Pathway富集分析。结果1．现有研究提示hsa-miR-17-92 cluster及其同源体在脂肪细胞分化、肿瘤疾病、心脏及肺发育、免疫系统与血管形成等生物学过程中有重要作用；2．hsa-miR-17-92 cluster及其同源体进化上高度保守，根据种子序列同源性可分为4类，且在多物种问非常保守；3．hsa．miR-17-92 cluster及其同源体预测靶基因的功能与细胞周期、细胞黏附、Wnt、TGF-β信号、p53、丝裂原活化蛋白激酶等信号通路有较大的相关性，可能参与了前列腺癌、胰腺癌、结肠癌等多种疾病通路。结论通过对hsa-miR-17-92 cluster及其同源体系统的生物信息学分析，初步阐明了hsa-miR一17-92 cluster及其同源体的基本生物学特征，并为hsa-miR-17-92 cluster后续研究提供了功能与机制的线索。

Objective To analyze the prediction of target genes of hsa-miR-17-92 clusters and the paralogs, so as to lay foundation and provide theoretical basis for the further studies of hsa-miR-17-92 cluster biological function in human preadipocytes and obesity development. Methods 1. All literatures concerning miR-17-92 clusters were searched in PubMed and Google;2. miRBase database was used to retrieve the sequence of hsa-miR-17-92 clusters according to the ＂seed sequence＂ to classify the member;3. National Center for Biotechnology Information（NCBI） blast, NCBI mapviewer and UCSC Genome Browser were used to analyze the characteristics of hsa-miR-17-92 clusters and the paralogs ;4. TargetScan5.1 ,PicTar and miRanda were used to predict target genes of hsa-miR-17-92 clusters, and the intersection of the 3 results as gene set was analyzed by Gene Ontologe（ GO ） analysis and Pathway analysis. Results 1. hsa-miR-17-92 clusters and the paralogs had been reported in adipocyte differentiation, cancer, heart and lung development, the immune system and angiogenesis, and involved in other biological process as well;2, hsa-miR-17-92 cluster members were highly conservative and divided into 4 categories ;3. the target genes set mostly existed in cell cycle, cell adhesion,Wnt signaling pathway,TGF-β signaling pathway, p53, mitogen-activated protein kinase（MAPK） and other signaling pathway and prostate cancer,pancreatic cancer and colon cancer and other diseases pathway. Conclusions The target genes set of hsa-miR-17-92 cluster enrich in multiple biological process for laying the foundation for the further study in human preadipocytes.