安徽省肥东县中华按蚊对4种常用杀虫剂抗性现状调查

Resistance assay of Anopheles sinensis to four conventional insecticides in Feidong County of Anhui Province

目的探明安徽省合肥市肥东县中华按蚊对二氯二苯三氯乙烷（滴滴涕，DDT）、马拉硫磷、残杀威与溴氰菊酯等4种常用杀虫剂的抗性现状。方法根据地理分布选择肥东县双桥村、赵坊村和管湾陈小郢3个自然村．于2012年7—8月以通宵牛诱法捕获饱血中华按蚊．采用WH0推荐的雌蚊接触筒法进行敏感性测试。每蚊种测定4～5筒．蚊虫总量〉100只，DDT、马拉硫磷、溴氰菊酯和残杀威的测试浓度依次为4％、5％、0．05％和0．1％，同时设立丙酮对照组。蚊虫接触供试杀虫剂60min，计算接触60min时的击倒率，计算半数击倒时间（KT50）。24h后记录蚊虫死亡数，计算死亡率，以击倒率和死亡率为测量指标。结果共捕获中华按蚊1576只。DDT、溴氰菊酯与残杀威对中华按蚊的击倒率为10．86％、9．21％和5．73％；DDT、溴氰菊酯、马拉硫磷和残杀威的校正死亡率分别为36．41％、7．54％、88．83％和29．45％。结论合肥市肥东县传疟媒介中华按蚊对DDT、残杀威、溴氰菊酯均产生高度抗性，对马拉硫磷产生轻度抗性。

Objective To determine the resistance situation of Anopheles sinensis to four conventional insecticides（DDT, malathion, propoxur and delatamethrin） in Feidong County of Hefei City. Methods Three villages （Shuangqiao, Zhaofang, and Chenxiaoying） of Feidong County were selected as the investigation spot according to historic malaria situation and the distribution of the imported Plazmodiun falciparum cases in recent years. Anopheles sinensis adults were collected by whole night cow-baited traps from July to August in 2012. WHO standard assays were used to test the resistance of Anopheles sinensis F0 generation female. Anopheles sinensis was tested for four to five test tubes, the amount of mosquitoes was larger than one hundred. The density of DDT, malathion, dehamerthrin, propoxur was 4%, 5%, 0.05% and 0.1%, respectively. Acetone was used as control at the same time. Knockdown rate was calculated after one hour insecticides exposure and mortality was recorded after 24 hours of exposure. Results Totally 1 576 Anopheles sinensis were captured. The resistance to 4% DDT, 0.05% dehamerthrin, 0.1% propoxur and 5% malathion was all detected in the population of Anopheles s inensis in Feidong County. The average knockdown rates of Anopheles sinensis to 4% DDT, 0.05% deltamerthrin, 0.1% propoxur were 10.86%, 9.21% and 5.73%, respectively. And the average adjusted mortality of Anopheles sinensis to 40 DDT, 0.05% dehamerthrin, 0.1% propoxur and 5% malathion was 36.41%, 7.54%, 88.83% and 29.45%, respectively. Conclusion The main malaria vector Anopheles sinensis in Feidong County showed dominant resistance to DDT, dehamethrin and propoxur, and primary resistance to malathion.