高效液相色谱法测定七叶灵颗粒中柚皮苷的含量

Determination of naringin in Qiyelin granules by high performance liquid chromatography

目的建立用高效液相色谱法测定七叶灵颗粒中柚皮苷含量的方法。方法使用KromasilC18色谱柱（250．0mm×4．6mm，5μm），流动相为乙腈：4％冰醋酸（17：83），检测波长为224nm，流速：1ml／min，柱温30℃。结果该色谱条件下显示能良好分离柚皮苷峰，避免杂质干扰，柚皮苷在0．08～2．00μg范围线性关系良好（r=0．9999），精密度相对标准偏差（RSD）：1．97％（n=5）。柚皮苷平均加样回收率为102．60％，RSD=1．86％（n=6）。结论用高效液相色谱法测定七叶灵颗粒中柚皮苷含量方法简便，重现性好，经过多批制剂检测，结果稳定，可用于七叶灵颗粒中柚皮苷的含量测定。

Objective To establish a high performance liquid chromatography method for determination na- ringin in Qiyelin granules. Methods Kromasil C18 column（250.0 mm × 4.6 mm, 5 μm） was used; the mobile phase was acetonitrile： 4% acetic acid （17： 83）. The detection wavelength was 224 nm; the flow rate was 1 ml/min and the column temperature was 30℃. Results This method determinated the naringin effectively and avoided the impurity. The linear range of naringin was at 0.08-2 μg （ r = 0. 9999 ） , relative standard deviation （RSD） was 1.97% （ n = 5 ）. The average recovery of naringin was 102.6% , RSD = 1.86% （ n = 6）. Conclusions The method of Qiyeling particles in naringin determined by high performance liquid chromatography is simple and repeatable. The result is stable. It can be used for determination the content of naringin in Qiyelin granules.