金黄色葡萄球菌的API-STAPH生化鉴定及核糖体分型分析(英文)

Identification of Staphylococcus aureus by API-STAPH test and ribosomal typing analysis

食源性致病金黄色葡萄球菌的筛查和检测备受关注,传统的生化鉴定方法存在耗时长和灵敏度低等缺陷,本文以原核生物核糖体小亚基为主要研究对象,利用16S核糖体RNA测序和核糖体基因分型技术对46株金黄色葡萄球菌进行了鉴定和分型,结果表明:API-STAPH生化方法鉴定的准确率为93%,而分子生物学鉴定的准确率≥99%;全自动微生物核糖体基因分型系统将46株金黄色葡萄球菌分为22个Ribogroup。上述研究将食源性金黄色葡萄球菌的鉴定上升到"亚型"的高度,16S核糖体RNA测序和核糖体分型技术可为食源性致病菌的风险监测提供有力的技术支持。

Staphylococcus aureus is one of the most prevalent pathogenic bacteria in food and beverages. Traditional method for S. aureus identification was based on biochemical test, which may lead to ambiguous result. In this article, API-STAPH test, 16S rRNA sequencing and ribosomal typing analysis were used for S. aureus identification and typing. The data suggested that the identification accurace by 16S rRNA sequencing（≥99%） is higher than that of API（93%）. Ribotyping divided the 46 strains into 22 Ribogroups. 16S rRNA sequencing and ribotyping analysis were more reliable for bacterial identification, and ribotyping analysis may play an important role in S. aureus subtype identification.