左氧氟沙星对厄贝沙坦药动学影响的研究

The influence of levofloxacin on the pharmacokinetics of irbesartan in rabbit

目的探讨左氧氟沙星（40mg／kg）诱导5天对新西兰大白兔体内厄贝沙坦药动学的影响。方法12只新西兰大白兔随机分为2组，分别是对照组[第1．5天，1％羧甲基纤维素溶液灌胃，第6天，厄贝沙坦（50mg／kg）灌胃）和左氧氟沙星诱导组（第1-5天，左氧氟沙星（40mg／kg）灌胃，第6天，厄贝沙坦（50mg／kg）灌胃]。采用高效液相．荧光检测法（HPLC-FD）测定厄贝沙坦血药浓度，DAS3．0软件进行数据处理，计算药动学参数。Q。，和己一为实验测得。主要药动学参数用SPSS13．0软件包进行统计分析。结果对照组和左氧氟沙星诱导组的主要药动学参数如下：AUC0-36分别为：（10．13±4．81）和（16．51±4．79）μg·h／mL;AUC0-∞分别为：11．97±5．67）和（20．43±7．44）μg·h／mL；CL分别为：（9．93±5．29）和（4．76±1．98）L／kg／h;Cmax分别为：（2．34±2．18）和（3．27±0．92）μg／mL；T1/2分别为：（4．75±1．09）和（6．46±5．70）h经统计分析，与对照组比较，左氧氟沙星（40mg／kg）诱导组对新西兰大白兔体内厄贝沙坦的主要药动学参数均无显著性差异（P〉0．05）。结论左氧氟沙星对新西兰大白兔体内厄贝沙坦的药动学无影响。

Objective To investigate the influence of levofloxacin on pharmacokinetics of irbesartan after oral administration levofloxacin 40mg/kg for 5 days. Methods 12 New zealand white rabbits were divided into 2 groups randomizedly as follow： control group and levofloxacin group. The concentrations of irbesartan were performanced with HPLC-fluorescence method. The results of the plasma samples were analysed with the DAS version 3.0（xacinBontz Inc., Beijing, China） software to determine the compartment models and the pharmacokinetic parameters, and the statistics were analysed by SPSS 13.0 software. Result The important pharmacokinetic parameters of irbesartan about control group and levofloxacin group were as the follows： AUC0.36were （10.13±4.81） and （16.5 1±4.79）μg.h/mL; AUC0-∞were （11.97±5.67） and （20.434±7.44）μg.h/mL; CL were （9.934±5.29） and （4.76±1.98） L/kg/h; C were（2.34±2.18） and（3.274±0.92） μg/mL; T,/z were （4.75±1.09） and （6.46q±5.70）h. Compared with the control group, there is not obvious influence on the pharmacokinetic parameters of irbesartan（P〉0.05） after inducing with levofloxacin 40mg/kg for 5 days .Conclusion There is not influence on the pharmacokinetics of irbesartan after inducing with levofloxacin for 5 days.