miRNA在不同药物治疗慢性乙型肝炎耐药患者血清中表达的变化

Change of miRNAexpression in serum of chronic hepatitis B patients treated with different antibiotics

目的观察miRNA在不同药物治疗慢性乙型肝炎耐药患者治疗期间血清中的表达变化,并分析其临床意义。方法选取2009年6月-2011年6月医院收治的50例慢性乙型肝炎治疗过程发生耐药的患者为研究对象,根据服用药物不同,分为核苷类似药治疗组和干扰素治疗组各25例,利用荧光定量技术系统分析所有患者在治疗期间血清中miRNA表达变化。结果 miRNA中let-7a、let-7f、miR-122、miR-223及miR-26a在核苷类似药治疗组患者治疗后的表达量分别为(22.12±2.12)、(22.34±3.17)、(22.92±3.14)、1(5.27±2.28)、(17.35±2.98)fmol/L,在干扰素治疗组患者治疗后的表达量分别为(21.31±2.05)、(21.23±2.12)、(21.72±2.18)、(13.87±2.17)、(16.28±1.87)fmol/L,两组治疗后各指标均高于治疗前和停药后,差异均有统计学意义(P<0.05);停药后与治疗前比较无明显差异;两组miR-602含量随治疗延续逐渐下降,比较差异有统计学意义(P<0.05);miRNA在干扰素治疗组耐药患者血清中的含量低于在核苷类似药治疗组中的表达,但变化趋势一致,分别比较差异无统计学意义。结论 miRNA在干扰素治疗组耐药患者血清中的含量低于在核苷类似药治疗组中的表达,但变化趋势一致。

OBJECTIVE To study the change of expression of miRNA in the serum of chronic hepatitis B patients treated with different antibiotics so as to analyze its clinical significance. METHODS A total of 50 cases of patients with chronic hepatitis B were selected as the research objects and were divided into the nucleoside analogues treatment group and the interferon treatment group with 25 cases in each according to the different drug treatment. The expression of rniRNA in the serum of all the patients during the treatment was determined by using fluorescence quantitative analysis technology. RESULTS After the treatment, the content of expression of let-7a,let-7f,miR- 122,miR-223 and miR-26a in the nucleoside analogues treatment group were （22. 12±2.12） , （22.34±3. 17）, （22.92±3.14）, （15.27±2.28） and （17.35±2.98） fmol/L, respectively, which were （21.31±2.05）, （21.23 ±2.12） ,（21.72±2.18）, （13.87±2.17） and （16.28±1.87） fmol/L in the interferon treatment group, all the indexes were higher after the treatment than before the treatment or after the drug withdrawal, the difference was statistically significant （P〈0. 05）; there was no significant difference between after the drug withdrawal and before the treatment; the content of miR-602 of the two groups decreased gradually as the treatment continued, the difference was statistically significant （P〈0. 05）. The content of rniRNA in the serum of the interferon treatment group was lower than that of the nucleoside analogues treatment group, but the change trend was the same, as compared separately, the difference was not statistically significant. CONCLUSION The content ofexpression of miRNA in the serum of the interferon treatment group is lower than that of the nucleoside analogues treatment group, but the change trend is the same.