HPLC法测定不同产地桂花中红景天苷和毛蕊花糖苷的含量

Determination of salidroside and verbascoside in Osmanthus fragrans from different producing areas by HPLC

目的:建立HPLC法测定不同产地桂花中红景天苷和毛蕊花糖苷的含量。方法:采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸水为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长220 nm,柱温为室温,进样量10μL。结果:红景天苷和毛蕊花糖苷的质量浓度分别在1.06～3.89μg.mL-1(r=0.9995,n=6)、8.84～32.4μg.mL-1(r=0.9997,n=6)内与峰面积呈良好的线性关系;平均回收率分别为98.3%和98.4%,RSD分别为1.1%和0.65%。通过比较不同产地桂花样品的测定结果,可知不同产地桂花中红景天苷和毛蕊花糖苷的含量有着较大差别。结论:该方法为各产地桂花药材进一步开发利用提供质量控制依据。

Objective：To establish an HPLC method for simultaneous determination of salidroside and verbascoside in Osmanthus fragrans produced from different places. Methods：The chromatographic separation was performed onan Agilent ZORBAX SB -C18 column（4.6 mm×250 mm,5μm）;the mobile phase was acetonitrile -0. 1% phosphoric acid solution with gradient elution at the flow rate of 1 mL·min^-1 ; the detection wavelength was 220 nm, thecolumn temperature was room temperature, and the injection volume was 10 μL. Results：The linear ranges were 1.06～3.89μg·mL^-1（r=0.9995,n=6） for salidroside and 8.84～32.4μg·mL^-1（r=0.9997,n=6） for ver-bascoside, respectively. The average recovery of salidroside was 98.3 %, RSD was 1.1% ; the verbascoside average recovery was 98.4% , RSD was 0. 65%. From the results obtained in this study,the contents of salidroside and verbascoside had great difference in Osmanthus fragrans collected from different places of origin. Conclusion： The method provides reference for the quality control for further development of Osmanthus fragrans in different produ-cing places.