摘要
目的:建立HPLC法测定不同产地桂花中红景天苷和毛蕊花糖苷的含量。方法:采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸水为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长220 nm,柱温为室温,进样量10μL。结果:红景天苷和毛蕊花糖苷的质量浓度分别在1.06~3.89μg.mL-1(r=0.9995,n=6)、8.84~32.4μg.mL-1(r=0.9997,n=6)内与峰面积呈良好的线性关系;平均回收率分别为98.3%和98.4%,RSD分别为1.1%和0.65%。通过比较不同产地桂花样品的测定结果,可知不同产地桂花中红景天苷和毛蕊花糖苷的含量有着较大差别。结论:该方法为各产地桂花药材进一步开发利用提供质量控制依据。
Objective:To establish an HPLC method for simultaneous determination of salidroside and verbascoside in Osmanthus fragrans produced from different places. Methods:The chromatographic separation was performed onan Agilent ZORBAX SB -C18 column(4.6 mm×250 mm,5μm);the mobile phase was acetonitrile -0. 1% phosphoric acid solution with gradient elution at the flow rate of 1 mL·min^-1 ; the detection wavelength was 220 nm, thecolumn temperature was room temperature, and the injection volume was 10 μL. Results:The linear ranges were 1.06~3.89μg·mL^-1(r=0.9995,n=6) for salidroside and 8.84~32.4μg·mL^-1(r=0.9997,n=6) for ver-bascoside, respectively. The average recovery of salidroside was 98.3 %, RSD was 1.1% ; the verbascoside average recovery was 98.4% , RSD was 0. 65%. From the results obtained in this study,the contents of salidroside and verbascoside had great difference in Osmanthus fragrans collected from different places of origin. Conclusion: The method provides reference for the quality control for further development of Osmanthus fragrans in different produ-cing places.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2013年第5期894-897,共4页
Chinese Journal of Pharmaceutical Analysis
基金
黑龙江省教育厅科学技术研究项目(12521559)
关键词
桂花
红景天苷
毛蕊花糖苷
苯丙素苷类
中药质量控制
高效液相色谱
Osmanthus fragrans
salidroside
verbascoside
phenylpropanoid glycoside
quality control for traditionalChinese medicine
HPLC