溶藻细菌NP23液体培养基的优化及生物安全性的研究

Optimization of liquid media and study of biological safety for algicidal bacteria NP23

【目的】为快速培养高浓度菌液以治理水体富营养化导致的藻类水华。【方法】首先用Plackett-Burman(PB)法筛选出影响NP23菌浓的3个重要因素KNO3、MnSO4.H2O和K2HPO4,然后对筛选出的重要因素在最佳值区域里用响应面方法(RSM)来优化NP23菌培养条件,最后用急性毒性试验和致突变试验对NP23菌液投入水体的生物安全性进行评价。【结果】优化后菌浓达到1013CFU/mL,比优化前菌浓大4个数量级。NP23菌液即使在大剂量下对鱼类也无急性毒性,对鱼类无致突变效应。【结论】研究结果为NP23菌实际应用提供参考。

[Objective] In order to cultivate high concentration of bacteria to deal with algal blooms caused by water eutrophication of algal blooms quickly. [Methods] Firstly, three important factors： KNO3, MnSO4？H2O and K2HPO4 which played important roles in NP23 bacteria concentration were selected based on the Plackett-Burman （PB） design experiments. Then, the best value of the selected important factors was picked out using response surface methodology （RSM） to optimize the process conditions of NP23. At Last, biological safety of NP23 bacteria liquid was evaluated by the acute toxicity and mutagenicity test. [Results] The bacteria concentration got 1013 CFU/mL after optimization, which were four orders of magnitude more compared with bacteria concentration before optimization and which showed that NP23 had no poisoning effect and no mutagenic effect on fish even in large doses. [Con-clusion] The results provide reference to practical applications of NP23.