结肠癌患者肿瘤组织及血清中Periostin蛋白表达的临床观察

Clinical Observation of the Expression of Periostin Protein in the Cancer Tissue and Serum of Patients with Colon Cancers

目的探索结肠癌患者血清和癌组织中Periostin蛋白的表达水平与结肠癌临床病理因素的相关性。方法选择64例结肠癌患者,获取其手术切除标本和血清,并和相应的癌旁组织和健康人血清进行了对照。应用免疫组织化学检测结肠癌组织Periostin蛋白的表达,应用酶联免疫吸附法检测了结肠癌患者和健康对照血清Periostin蛋白的表达。所有结肠癌患者按照病理临床因素分类,包括性别、结肠癌TNM分期、浸润深度、有无淋巴结转移、病理分级、复发转移及手术前后。结果结肠癌患者血清Periostin蛋白水平显著高于健康人群,癌组织Periostin蛋白的表达阳性率也显著高于癌旁组织(P<0.05)。血清及癌组织Periostin的表达与TNM分期、浸润深度、有无淋巴结转移、肿瘤复发转移有关,与性别和病理分级无关。手术能显著降低血清Periostin蛋白水平。结论结肠癌患者Periostin蛋白血清浓度和癌组织表达显著高于健康人群,且与结肠癌的浸润转移成正相关。血清Periostin蛋白是一种预测结肠癌的侵袭、转移、手术根除程度和复发的潜在标志。

Objective To explore the expression of periostin protein in cancer tissue and serum, and their relationship with clinicopathologic factors in colon cancer patients. Methods The surgical specimens and serums from 64 patients with colon cancer were selected and compared with the corresponding control specimens from healthy people. Immunohistochemistry was performed to detect the expression of periostin protein in colon cancer tissues, and enzyme-linked immunosorbent assay （ELISA） was performed to detect serum periostin protein expression in colon cancer patients and healthy controls. All cancer patients were classified according to elinicopathologic factors, including gender, TNM stage of colon cancer, depth of invasion, lymph node metastasis, histological grade, metastasis and recurrence, and surgery. Results The average concentration of serum periostin in patients with colon cancer was significantly higher than that in healthy population （P 〈0.05）. The expression of periostin protein in cancer tissue was also significantly higher than the adjacent tissues. Serum and tumor tissue periostin expression were both related with TNM stage, depth of invasion, lymph node metastasis, tumor recurrence and metastasis. The periostin expression levels were related with lymph node metastasis and tumor invasion depth. No significant correlation was found in periostin protein expression with tumor grade or gender. Surgery significantly reduced serum levels of periostin protein. Conclusions The expressions of periostin protein in serum and cancer tissue in patients with colon cancer were significantly higher than those in healthy people, and were positively related with invasion and metastasis of colon cancer. Serum periostin protein is a potential prediction marker for invasion, metastasis, extent of eradication by surgery and recurrence in colon cancer.