116例多发性骨髓瘤免疫特征表型分析

Analysis of immunophenotypic characteristics in 116 cases of multiple myeloma

目的了解多发性骨髓瘤(MM)的免疫表型特征,以及CD45、CD19与临床特征的相关性。方法采用三色流式细胞术对116例初诊MM病例进行免疫表型分析,并收集患者的实验室和临床指标。结果 116例MM均表达CD38,其他抗原CD19、CD138、CD56、CD20、CD33、CD117、CD45的阳性率分别为10.3%、96.6%、60.7%、3.4%、70.0%、25.6%、17.2%,无1例表达CD7、CD34。在116例MM中,胞浆κ轻链阳性率为34.5%,λ轻链阳性率为48.3%。CD45阳性患者分期较好,其表达缺失患者易出现骨破坏,微球蛋白、C反应蛋白水平较高;CD19表达与高分期有关,易出现贫血,微球蛋白、C反应蛋白水平较高。结论了解MM的免疫表型特点对此类疾病的诊断具有重要意义,其中CD45、CD19与分期等临床生物学特征有关,有助于判断预后。

Objective To investigate the immunophenotypic characteristics of 116 cases of multiple myeloma （MM） and the relation between the immunophenotype and clinical features. Methods Immunophenotyping was per formed in 116 patients of MM by three color multiparameter flow cytometry analysis using a panel of monoclonal an- tibodies. Results In 58 cases of MM, all cases expressed CD38, the positive ratios of other antigen such as CD19, CI）138,CD56,CD20,CD33,CDl17,CD45 were 10.3~//oo,96. 6%,60. 7~/00,3.4%,70. 00/00,25. 6%,17.2 ~.~0, respective- ly. None of the MM cases expressed CD7 ,CD34. Lack of CD45 expression was associated with greater frequencies of destruction of bone, higher levels of t/12--microglobulin and C reaction protein （CRP）. Expression of CD19 was asso- ciated with higher frequency of advanced international staging system stages, higher levels of 62 -- microglobulin and CRP, greater frequencies of anemia. Conclusion The expression of CD45 and CD19 could be important clinical prog- nosis value for MM, which might be helpful for identifying the prognostic factors and guiding clinical treatment in MM.