脐血间充质干细胞在造血干细胞移植中的临床应用

The clinical application of umbilical cord blood mesenchymal stem cell in peripheral hematopoietic stem cell transplantation

目的研究联合间充质干细胞和造血干细胞移植后的临床效果。方法实验组为联合移植的病例7例,对照组为外周造血干细胞移植的病例8例,通过分别观察每名患者的血小板重建时间、白细胞重建时间、住层流病房时间以及移植前后症状的改善情况、感染情况、GVHD发生率以及1年存活率来进行统计分析,判断2组的移植效果。结果实验组与对照组病例血小板重建平均时间分别为8.714、19.500 d,P<0.01;粒系重建平均时间分别为12.143、19.500 d,P<0.05;住层流病房平均时间分别为23.857、36.750 d,P<0.01;感染发生率分别分别为28.57%、62.50%;GVHD发生率为0、37.50%;一年内死亡率分别为0、12.5%。结论联合脐血间充质干细胞移植对造血干细胞移植后的归巢、发育、分化、成熟以及移植后的造血重建都有促进的作用,有助于减少移植后患者的感染率、GVHD发生率及死亡率,是造血干细胞移植技术的一步大跨越。

Objective To study the clinical efficacy of cotransplantation of umbilical cord blood mesenchymal stem cells and peripheral hematopoietic stem cells. Methods Experimental group included seven patients who received cotransplanta- tion with Umbilical cord blood mesenchymal stem cells and peripheral hematopoietic stem cells. Control group included eight patients only received transplantation with peripheral hematopoietie stem cell. We observed the times to neutrophil and platelet engraftment, the time spend in laminar flow room, the symptom, infection, the incidence of GVHD and the survival rate of one year after transplantation. Rusults The times to platelet engraftment of the experimental group and the control group was 8. 714 and 19. 500 days （P 〈 0. 01 ）, the times to neutrophilengraftment was 12. 143 and 19. 500 days （P 〈 0.05 ）, the times spend in the laminar flow room was 23. 857 and 36.750 days （ P 〈 0. 01 ） , the incidence of infection was 28. 57% and 62.50% ,the incidence of GVHD was 0 and 37.50%, and the mortality rate of one year was 0 and 12.5%. Conclusion Cotransplantation with UCMSC would improve the efficaey of pe- ripheral hematopoietic stem-cell transplantation.