鸡粪与四环素对土壤脲酶和磷酸酶活性的影响

Effect of Chicken Manure and Tetracycline on Soil Urease and Phosphatase Activity

采用室内土壤培养方法,分别在培养第1、15、30、45、60、75 d采样,测定土壤脲酶和磷酸酶活性,研究鸡粪、四环素和两者共存对土壤脲酶和磷酸酶活性的影响。结果表明:(1)土壤中添加鸡粪能够明显激活两种土壤酶的活性,鸡粪对土壤脲酶激活时间大于75 d,对磷酸酶的激活时间为60 d左右,最高激活率分别为287.7%和69.0%。(2)土壤中加入0.06 mg.kg-1四环素可激活土壤脲酶与磷酸酶的活性,最大激活率出现在培养第15、45 d,激活率分别为22.6%、23.6%,加入浓度为0.12 mg.kg-1和0.24 mg.kg-1四环素在整个培养过程中抑制两种土壤酶活性。(3)在土壤中同时加入鸡粪和不同浓度四环素,各处理组土壤两种酶活性均表现出激活作用,激活率均高于100%。由此可知,在四环素污染的土壤中加入鸡粪能够增强土壤脲酶和磷酸酶活性,降低四环素对土壤脲酶和磷酸酶活性的影响。

In this study,the effects of chicken manure and tetracycline on soil urease and phosphatase activities were investigated in a laboratory incubation.Soil sample were collected at 1,15,30,45,60 days and 75 days of incubation.Chicken manure applied to the soil activated significantly(P<0.05),the activities of soil urease and phosphatase with the maximum activating ratio of 287.7% and 69.0% at 75 days and 60 days of incubation,respectively.The concentration of 0.06 mg·kg-1 tetracycline activated the activities of soil urease and phosphatase with the maximum activating ratio of 22.6% and 23.6% at 15 days and 45 days of incubation,respectively.And the concentration of 0.12 mg·kg-1 and 0.24 mg·kg-1 tetracycline inhibited the two kinds of soil enzymes activities throughout the incubation.The incorporation of chicken manure and various concentrations of tetracycline activated soil enzymes activities with the activation ratio more than 100% for both of the two soil enzymes.The results showed that chicken manure added to the soil contaminated by tetracycline could enhance the activities of soil urease and phosphatase enzyme and control the effects of tetracycline on soil urease and phosphatase activities.