杂交猪IFN-γ和IL-18双基因在Rosetta TM大肠杆菌的表达

Construction and expression of the prokaryotic expressing vector containing IFN-γ and IL-18 gene of inbred pig

依据已测的杂交猪IFN-γ、IL-18基因的核苷酸序列,设计4条引物,通过重叠PCR的方法将2个基因进行连接,并在两个基因连接处引入45bp的连接肽的核苷酸序列;融合基因通过pEGX-4T-1载体及RosettaTM表达菌实现了原核表达,蛋白可被GST标签多抗识别。本研究的进行,为IFN-γ/IL-18融合蛋白的功能研究奠定了基础。

Based on the reported gene sequences of IFN-γ and IL-18, four primers were designed to get a fusion gene which was made of genes of IFN-γ and IL-18 by overlap PCR. There was a 45 bp size of linker peptide between of the fusion genes of IFN-γ and IL-18. By RosettaTM E. coil,fusion gene which was inserted into pEGX-4T-1 vector,was expressed with a GST tag. In this study,we express the protein of fusion gene of IFN-γ and IL-18,which will be the basis of further studying the function of the fusion protein.