摘要
为揭示中药香芪汤及其有效成分的体外抗炎作用机理,利用ELISA法研究了不同质量浓度(0.2、1、5mg/L)的香芪汤对细胞因子TNF-α分泌的影响,并运用Western blot法检测了香芪汤及其有效成分黄芪甲苷、α-香附酮和穿心莲内酯对ERK1/2和JNK1/2的影响。结果显示,1、5mg/L质量浓度的香芪汤能够极显著抑制RAW264.7巨噬细胞TNF-α的分泌(P<0.01),香芪汤及其有效成分能够极显著降低JNK1/2和ERK1/2的磷酸化水平(P<0.01)。由此推论,中药香芪汤及其有效成分可能通过抑制炎症因子的分泌,降低MAPK信号通路的活性发挥其抗炎作用。
To reveal the anti-inflammation mechanism of Xiang-Qi-Tang and its active components of Chinese herbal formula in vitro, We determined different concentrations of Xiang-Qi-Tang（0. 2,1,5 mg/L） on the influence of secretion of TNF-α by ELISA as well as the influence of Xiang-Qi-Tang and its active components including astragalosideIV, α-cyperone and andrographolide on the ERK1/2 and JNK1/2 via Western blotting. The results showed that 1 and 5 mg/L of Xiang- Qi-Tang can significantly inhibit the secretion of TNF-α of RAW264.7 macrophages（P〈0.01）, meanwhile,Xiang-Qi-Tang and its active components can memorably reduce the phosphorylation level of ERK1/2 and JNK1/2（P〈O. 01）. The results indicated that Xiang-Qi-Tang and its active components may play an anti-inflammation activity by mean of the reduction of mediator of inflammation and the attenuation of the activation of MAPK signal pathway.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第5期776-779,共4页
Chinese Journal of Veterinary Science
基金
北京农学院兽医学(中医药)北京市重点实验室开放课题(TCVM-201104)
中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省新兽药工程重点实验室开放课题(SYSKF2011KT05)
关键词
中药
香芪汤
炎症
MAPK
Chinese medicine
Xiang-Qi-Tang
Inflammation
MAPK
