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荧光定量分析猪睾丸组织中miR-34b的表达规律 被引量：3

Analysis of miR-34b expression in swine testicular tissues by RT-PCR
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摘要 运用荧光定量分析方法检测军牧1号白猪不同发育阶段睾丸组织miRNA-34b的表达量差异,结果:miRNA-34b在3月龄前的表达量都非常低,从5月龄开始表达量呈明显上升,7月龄达到峰值后开始明显下降。在猪的发育过程中,miRNA-34b的表达趋势曲线呈先增后降。结果表明miRNA-34b可能与猪雄性生殖发育相关。 MicroRNAs （miRNAs） are short （-22 nucleotides） non-coding RNA molecules that play important roles by repressing translation or cleaving RNA transcripts. Some reports showed miRNA-34b is an important regulator in germ cell differentiation process of male humans and mice. We detected the different expressions of miR-34h of testicular tissue by real time quantitative PCR （RT-PCR） in Junmu No. 1 white pig among the whole developmental stages. The results showed the expressions of miRNA 34b keep very low level before 3-month-old, rising dramatically from 5-month old,reaching a peak at 7 month old,and then descend apparently. Overviev the expression trend of miRNA-34b in the whole sexual development processes,it runs a rule that first rising and then descending. The conclusion is anticipated that miR 34b keep a high possibility correlation with sexual development of male pig.
作者 赵伟 李传民 高峰 张晓军 王大力 白春燕 孙博兴
机构地区 吉林大学畜牧兽医学院
出处 《中国兽医学报》 CAS CSCD 北大核心 2013年第5期785-787,794,共4页 Chinese Journal of Veterinary Science
基金 吉林省科技发展计划项目(20120208) 吉林大学基本科研业务费项目
关键词 miRNA-34b 军牧1号白猪 荧光定量 miRNA 34b Junmu No. 1 white pig real time quantitative PCR
分类号 Q78 [生物学—分子生物学]
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参考文献19

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同被引文献48

  • 1孙亚平,严缘昌,张尚权,赵峰.大鼠和小鼠睾丸表皮生长因子表达的免疫组织化学定位观察[J].中国组织化学与细胞化学杂志,1997,6(1):19-23. 被引量：11
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中国兽医学报
2013年 第5期

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