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NGF对输卵管黏膜上皮细胞促性腺激素受体表达的影响 被引量：3

NGF affects the expression of gonadotropin receptors in oviduct epithelial cells
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摘要 本研究以牛输卵管黏膜上皮细胞为研究对象,利用荧光定量PCR方法检测NGF和酪氨酸激酶抑制剂K252α对输卵管粘膜上皮细胞膜促性腺激素受体FSHR和LHRmRNA表达的影响。添加30、60、100μg/L NGF可以显著增加FSHR mRNA的表达,添加30和60μg/L的NGF同样增加LHR mRNA的表达。添加抑制剂K252α后,LHR和FSHR的表达量均比对照组显著下降。该结果说明NGF可以通过其受体调节输卵管黏膜上皮细胞促性腺激素受体FSHR和LHR的表达。 We investigated the effects of NGF and K252a on the expression of gonadotropin recptors（FSHR and LHR） in cow oviduct epithelial cells with q-PCR. The expression level of FSHR in cells cultured media with NGF（30,60 or 100 μg/L） were significantly higher than that in the control. The expression level of LHR mRNA in cells cultured with NGF （30 or 60μg/L） were also significantly higher than that in the control. However,K252a could decreased the expression of FSHR and I.HR.
作者 刘立文 马永和 李万宏 王春强 周虚
机构地区 河北北方学院动物科技学院 吉林大学畜牧兽医学院
出处 《中国兽医学报》 CAS CSCD 北大核心 2013年第5期795-798,共4页 Chinese Journal of Veterinary Science
基金 十二五国家科技支撑计划项目(2011BAD19B02)
关键词 输卵管 神经营养因子 促性腺激素受体 Oviduct, NGF, Gonadotropin Receptor
分类号 S852.1 [农业科学—基础兽医学]
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参考文献10

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二级参考文献27

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共引文献19

同被引文献54

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引证文献3

二级引证文献8

中国兽医学报
2013年 第5期

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