低温春化对菘蓝体内碳氮代谢的影响

Effect of low-temperature vernalization on metabolism of carbon and nitrogen of Isatis indigotica

目的:探索菘蓝在低温春化过程中碳、氮代谢的变化。方法:以云南菘蓝和北京菘蓝6叶龄幼苗为实验材料,4℃条件下进行低温处理25 d,并测定碳氮代谢相关指标。结果:低温春化使菘蓝体内的可溶性糖、还原糖、可溶性蛋白质含量呈上升趋势,春化完成时达到最大值,然而低温春化使菘蓝体内的淀粉、全氮含量呈下降趋势,春化完成时降至最低。结论:低温春化过程中,高的C/N值才能促进菘蓝春化。

Objective： The aim of the present study is to investigate the effect of low temperature vernalization on metabolism change of carbon and nitrogen of Isatis indigotica. Method ： The Yunnan and Beijing I. indigotica seedlings with six leaves were vernalized at 4 ℃ for 25 days, and the metabolism indicators of carbon and nitrogen were measured. Result： There appeared a dramatic increase in the soluble sugar content, reducing sugar content and soluble protein content in response to the low temperature, after termination of vernalization it reached the maximum, however, starch and total nitrogen concentration decreased significantly, after termina- tion of vernalization it reached the minimum. Conclusion ： The high C/N value can promote the low temperature vernalization of I. indigotica.