利用胚抢救技术获得沙田柚×枸橼杂交后代及其SRAP检测

Acquisition of hybrids of Pummelo×Citron by using embryo rescue and their identification by SRAP molecular markers

【目的】为了获得沙田柚×枸橼杂交苗,【方法】以沙田柚为母本,枸橼为父本进行杂交育种,利用胚抢救技术获得杂种后代,并利用SRAP分子标记对亲本和子代的DNA进行扩增,分析亲本与杂交种之间扩增条带的差异。【结果】结果表明,最终获得27株杂交后代,引物组合Me6-Em5和Me10-Em12可鉴定其中5株为有性后代。【结论】MT+GA3(1 mg.L-1)培养基适合沙田柚×枸橼杂交胚抢救,利用SRAP标记可鉴定出杂交子代,可为以后利用BSA法构建抗感病池寻找柑橘抗溃疡病相关基因打下基础。

[Objective] In order to obtain hybrid seedlings of pummelo citron, [Method]Shatian pummelo as female parent and citron as male parent were used in cross breeding program. Embryo rescue technology was used for embryo growth, and sequence-related amplified polymorphism（SRAP） molecular maker was used to identify parents（Shatian pummelo and Citron ）and theirs hybrids according to the polymorphism. [Result] Results showed that twenty-seven hybrids were finally got and five of them were identified by primer combinations Me6-Em5 and Mel0-Eml2 finally. [Conclusion]MT＋GA3 （1 mg-L-1） was suitable for hybrid embryo rescue and SRAP makers could be used to indentify them. The real hybrids could be used to build resistant and diseased pool by BSA and to find related genes resistant to citrus canker.