太极拳运动后白细胞Th1/Th2型细胞因子mRNA的表达

Effects of Tai-chi on Th1/Th2 type cytokine mRNA expression in white blood cell

目的:探讨太极拳运动诱导外周血白细胞Thl/Th2型细胞因子mRNA表达情况,以进一步了解太极拳运动对Th1/Th2型细胞因子平衡的影响,探讨太极拳健身的可能免疫学机制。方法:在健康中老年女性学练太极拳前、练习3月和6月时对其外周血白细胞自发性Thl/Th2型细胞因子mRNA表达进行实时定量观测。结果:经过太极拳运动干预,中老年妇女外周血白细胞INF-γmRNA的表达量逐渐降低,3、6月时表达量均与干预前相比存在明显差异;IL-4 mRNA表达量先降低后回升,但仍未达初始水平,且3月时的表达量与干预前相比存在显著性差异;INF-γmRNA与IL-4 mRNA比值逐渐降低,与干预6月相比干预前和干预3月均存在明显差异。结论:太极拳运动使健康中老年妇女Th1/Th2平衡向Th2极化,提示太极拳运动调节机体Thl/Th2型细胞因子的平衡也许是太极拳调节中老年机体免疫力、增强健身效果的可能机制。

Objective： To study whether Tai -chi can effectively induce the expression of Thl/Th2 type cytokine messenger RNA （mRNA） in white blood cell. Methods ： Twenty - one community - dwelling middle - aged and old women participated in an intervention program of supervised 48 - style Tai -chi for lh per time, 3 times per week for 24 weeks. Thl/Th2 type cytokine mRNA ex- pression of white blood cell was detected by reverse transcription - polymerase chain reaction （ RT - PCR） before intervention, at 12 wk and 24 wk of training. Results： （1） The expression of INF-~ mRNA both decreased at 12 wk （P 〈 0.05 ） and 24wk （P 〈 0.05 ） compared with that of before in- tervention respectively. （2） The expression of IL- 4 mRNA decreased at 12wk and then increased at 24wk,and there are significant differences only when between 12 wk and before intervention. （3） The ratio of INF - r mRNA and IL - 4 mRNA decreased gradually after Tai - ehi intervention, andthere are significant differences both before intervention and at 12 wk compared with at 24wk. Con- clusion ： The Th2 cytokine in white blood cell increased and predominated after Tai - chi intervention and it suggests that Thl/Th2 cell balance has important implications for the health mechanism of Tai - chi exercise for middle - aged and old women.