电针“三阴”穴对自身免疫性前列腺炎大鼠炎症因子的影响

Decreasing effect of electro-acupuncture at Sanyin points on inflammatory cytokines in autoimmune prostatitis rats

目的:观察电针"三阴"穴对自身免疫性前列腺炎大鼠局部炎症因子的影响。方法:在大鼠皮内注射同种异体Wistar雄性大鼠前列腺蛋白提纯液,辅以双重免疫佐剂复制自身免疫性前列腺炎大鼠模型。将40只雄性大鼠随机分为正常对照组、模型组、舍尼通对照组、电针组,每组10只。除正常对照组外,其余大鼠均造模处理,45 d后,正常对照组与模型组以抓取、捆绑、固定处理,电针组和舍尼通组分别给予电针"三阴"穴治疗和舍尼通药物灌胃。连续治疗2个疗程(每个疗程7 d,间隔1 d,共15 d)后处死各组动物,检测各组动物前列腺组织中肿瘤坏死因子α(TNF-α)、一氧化氮合酶(iNOS)、总抗氧化能力(T-AOC)及丙二醛(MDA)水平。结果:与正常对照组比较,模型组前列腺组织中TNF-α表达显著升高[(32.20±1.65)pg/ml vs(15.31±1.36)pg/ml](P<0.01)、iNOS活性显著增强[(1.25±0.23)U/ml vs(0.81±0.33)U/ml](P<0.01),T-AOC活性显著减低[(1.11±0.15)U/ml vs(1.56±0.16)U/ml](P<0.01),MDA含量明显增高[(0.91±0.21)nmol/ml vs(0.66±0.14)nmol/ml](P<0.05);与模型组比较,电针组前列腺组织中TNF-α表达[(17.32±2.69)pg/ml]明显降低(P<0.01)、iNOS活性[(0.98±0.15)U/ml]显著减低(P<0.05),T-AOC活性[(1.44±0.26)U/ml]明显增加(P<0.05),MDA含量[(0.70±0.20)nmol/ml]明显减低(P<0.05)。结论:电针"三阴"穴能够降低促炎症细胞因子活性,降低血管通透性,减少炎症细胞侵润,并提高局部抗氧化防御系统活性,从而抑制前列腺组织形态结构的损伤,减轻炎症反应,达到治疗目的。

Objective： To observe the changes of inflammatory cytokines in autoimmune prostatitis （AIP） rats treated by electro-acupuncture （EA） at Sanyin points. Methods： We selected 40 Wistar male rats in this study, 10 as normal controls, and the other 30 made AIP models by intradermal injection of protein purification liquid from the prostate of allogeneic male rats with dual immune adjuvant. Then we randomly divided the AIP models into a model, a Cernilton control and an EA group of equal number, the latter two groups treated by Cernilton enema and EA, respectively. After 15 days of treatment, all the animals were sacrificed for detec tion of the levels of TNF-α, iNOS, MDA and T-AOC in the prostate tissue. Results ： Compared with the normal controls, the model rats showed significantly elevated TNF-α expression （ [ 15.31 ± 1.36] vs [32.20 ± 1.65] pg/ml, P 〈0. 01 ）, iNOS activity （ [0.81 ± 0.33 ] vs [ 1.25 ± 0.23 ] U/ml, P 〈 O. 01 ） and MDA content （ [ 0.66 ± 0.14 ] vs [ 0. 91 ± 0.21 ] nmoL/ml, P 〈 0. 05 ）, but markedly reduced T-AOC activity （ [ 1.56 ±0.16] vs [ 1.11 ±0.15] U/ml, P 〈0.01）. In comparison with the model group, the EA group exhibited significantly reduced levels of TNF-α （ [ 17.32 ± 2.69 ] pg/ml, P 〈 0. 01 ）, iNOS （ [ 0.98 ± 0.15 ] U/ml, P 〈 0.05 ） and MDA （ [ 0.70 ± 0. 20 ] nmol/ml, P 〈 0. 05 ）, but remarkably increased level of T-AOC （ [ 1.44 ± 0.26 ] U/ml, P 〈 0. 05 ）. Conclusion： Electro-acupuncture at Sanyin points can protect the prostate tissue from morphological damage and reduce inflammatory reaction by decreasing pro-inflammatory cytokine activity, vascular permeability and inflammatory cell infiltration and increasing the activity of the antioxidant defense system.