白细胞介素1α mRNA在神经胶质瘤中的表达及意义

Expression and its significance of interleukin-1α mRNA in gliomas

目的定量检测白细胞介素1α（IL-1α）mRNA在神经胶质瘤组织和正常脑组织中的表达，并探讨其与临床特征的关系。方法运用实时荧光定量PCR检测31例神经胶质瘤组织和22例正常脑组织中IL-1α mRNA的表达，分析其与临床特征的相关性。结果IL-1α mRNA在神经胶质瘤组织中的相对表达量为0．10，在正常组脑组织中为0．01，两者差异具有统计学意义（U=207．0，P=0．016）。IL-1α mRNA在WHO Ⅲ-Ⅳ级神经胶质瘤中的相对表达量为0．72，在Ⅰ-Ⅱ级中为0．02，两者差异具有统计学意义（U=36．0，P=0．001）；而IL-1α RNA相对表达量在性别和年龄之间差异无统计学意义（P〉0．05）。结论IL-1α mRNA在神经胶质瘤组织中呈显著高表达，其可能在神经胶质瘤的恶变过程中发挥重要作用。

Objective To quantitatively detect the expression of interleukin-1α （IL-1α） mRNA in glioma tissues and normal brain tissues, and explore its relation to clinicopathological features. Methods The expression of IL-1α mRNA was detected in 31 glioma tissues and 22 normal brain tissues by real-time PCR, and the relationship between IL-1α mRNA and clinical characteristics was also analyzed. Results The relative expression levels of IL-1α mRNA in glioma tissues was 0.10, while 0.01 in normal tissues, and there was a significant difference between them （U = 207.0, P = 0.016）. Additionally, the expression of IL-1α mRNA in glioma patients with pathological WHO grades Ⅲ to Ⅳ was 0.72, and 0.02 in grades Ⅰ to Ⅱ, and there was statistical significance in difference between them （U = 36.0, P = 0.001）. However, the IL-1α mRNA level was not related to the gender and age of glioma patients （P 〉 0.05）. Conclusion IL-1α mRNA can present with significantly higher expression in glioma tissues, indicating that IL-1α may play a critical role in the progression of tumorigenesis.