人SFRP1基因及其编码蛋白基本特性及抗原表位的生物信息学分析

Bioinformatics analysis of the structure and epitope of gene encoding SFRP1 protein from human

目的:通过生物信息学分析人分泌型卷曲相关蛋白1(SFRP1)基因编码蛋白的主要特性与抗原表位,筛选该蛋白的T/B细胞联合表位。方法:利用蛋白分析专家系统(ExPASy)提供的ProtParam、SignalP、SO-SUI、MotifScan、NCBI上的ORF finder、SYFPEITHI、NetCTL等生物信息学在线分析程序,结合Gene Runner、DNAMAN、DNAStar等生物信息学软件,分析、预测SFRP1蛋白的理化性质、信号肽、可溶性、表面可及性、可塑性、跨膜区、翻译后修饰位点、亲(疏)水性、二级结构、T/B细胞抗原表位等。结果:该蛋白由314个氨基酸组成,分子式为C1588H2516N426O436S26,分子质量单位为35.3856kD,等电点理论值为9.10,波长280nm时的吸光度(A)值为1.029,半衰期为30h,有16个潜在B细胞表位、2个CTL表位和辅助性T细胞联合表位、3个T/B细胞联合表位,为可溶性表达蛋白。结论:SFRP1基因编码的SFRP1为可溶性表达蛋白,有3个T/B细胞联合表位,可作为人类多种肿瘤免疫学治疗的重要靶点。

Objective：To study the main characteristics of the SFRP1 protein from human （SFRP1） encoding by SFRP1 gene and its T/B cell epitopes by bioinformatics. Methods：The physico -chemical property, solubility, surface accessibility, flexibility, post - translational modification sites, signal peptide, transmembrane domain, hydrophilicity/ hydrophobicity, secondary structure and T/B cell epitopes of SFRP1 protein were analyzed and predicted by using the bioinformatics tools ExPASy, including ProtParam, SOSUI, Moti/Scan, ORF finder and SignalP, combined with bioinformatics softwares （ Gene Runner, DNAMAN, DNAStar, etc）. Results： SFRP1 protein consisted of 314 amino acids with listed characters ： the molecular formula, C1588H2516 N426 O436S26; the molecular mass ,35. 3856 kD ; the value of theoretical isoelectric point 9.10 ; the absorbance （A） value 1. 029 （280nm） ; half - life is 30hours. There were 16 potential B cell epitopes, 2 T cell epitopes and 3 conjunctive cell epitopes as soluble protein expression. Conclusion： The SFRP1 protein encoding by SFRP1 gene can be used for human cancer immunology therapy because of its soluble protein expression and conjunctive cell epitopes.