miR-21在外阴鳞癌组织中的表达及临床意义

Expression and clinical significance of miR-21 in vulvar squamous cell carcinoma

目的:探讨微小RNA-21(miR-21)在外阴鳞癌(vulvar squamous cell carcinoma,VSCC)组织中的表达及临床意义。方法:采用茎环实时荧光逆转录聚合酶链反应(real-time RT-PCR)检测25例外阴鳞癌组织、30例外阴上皮内非瘤样病变组织(外阴增生性营养不良15例、外阴萎缩性营养不良15例)及10例正常外阴组织标本中miR-21的表达。结果:miR-21在外阴鳞癌组织中的相对表达量为(5.505±2.163),明显高于外阴上皮内非瘤样病变组织(2.520±1.260)及正常外阴皮肤组织(1.478±0.601)(P<0.05),而miR-21在外阴上皮内非瘤样病变组织及正常外阴组织间表达量无明显差异。在不同临床分期、不同组织学分级的外阴鳞癌组织中miR-21表达量有统计学差异(P<0.05)。结论:外阴鳞癌组织中miR-21的表达升高,提示miR-21可能在外阴鳞癌的发生发展中发挥致癌基因的作用。

Objective：To investigate expression and clinical significance of miR -21 in vulvar squamous cell carcinoma. Methods：The expression of miR -21 was detected by stem -loop real -time RT -PCR in 25 cases of vulvar squamous cell carcinomas,30 cases of nonneoplastic epithelial disorders of vulvar and 10 cases of normal vulvar tissues. Results： The relative expression level of miR - 21 （ 2 ^- △△CT ） was （ 5. 505 ± 2.163 ） in the vulvar squamous cell carcinomas, significantly higher than that in the nonneoplastic epithelial disorders of vulvar （2. 520 ± 1. 260）and normal vulvar tissues（ 1. 478 ~ 0. 601 ） （P 〈 0.05 ）, but comparable between the latter two groups. And the expression in vulvar squamous cell carcinoma tissues was significantly associated with the pathological grading and staging. Conclusion ：The increased expression of miR- 21 in vulvar squamous cell carcinoma suggests that it might play a role as an oncogene in the tumorigenesis and development of vulvar squamous cell carcinoma.