P27kip1在结直肠癌中的表达及临床意义

The expression of P27kip1 in colorectal cancer and its clinical significance

目的:通过检测结直肠癌病人肿瘤组织及癌旁组织中P27kip1的表达,探讨其与结直肠癌患者预后的相关性。方法:应用免疫组织化学方法检测62例结直肠癌患者癌组织及癌旁组织中P27kip1的表达水平,应用SPSS19.0软件分析其表达差异及与临床病理特征及预后的相关性。结果:癌旁组织P27kip1高表达率为62.9%,与正常组织中的P27kip1基因表达无统计学意义,而癌组织中P27kip1高表达率为37%;P27kip1的表达水平与年龄、性别、肿瘤位置、肿瘤分化无明显关联(P>0.05),但与肿瘤的病理分期及有无淋巴转移有关(P<0.05);单因素生存分析提示P27kip1高表达的病人生存时间较长(P<0.05),然而通过COX比例风险模型进行多因素分析,没能提示P27kip1的表达能作为独立预测生存的因素。结论:P27kip1蛋白的高表达可以作为结直肠癌的预后评估的重要参考指标,但不能作为独立预测预后的指标。

Objective：To detect the expression of P27kipl in eoloreetal cancer,and investigate the relationship between the P27kipl expression and prognosis. Methods： The expression of P27kipl was detected by immunohistochemistry in surgically resected specimens from 62 patients colorectal cancer,and the relationship with the elinieopathological features and prognosis was analyzed. Results： The high expression rate of P27kipl was 62.9% in adjacent noncancerous tissues,which showed no significant difference with the expres- sion of P27kipl in normal tissues. The high expression rate of P27kipl in tumor tissues was 37% ,and the expression of P27kipl corre- lated with TNM stage and lymph metastasis （P 〈 0.05 ） , but not with age, sex, tumor location and tumor differentiation （ P 〉 0.05 ）. Univariate survival analysis showed that patients with higher expression of P27 survived for longer periods of time （ P 〈 0.05 ） , but mul- tivariate analysis with COX proportional hazards model showed that P27kipl can not be used as independent predictors of survival fac- tors in colorectal cancer. Conclusion： The high expression of P27kipl protein in coloreetal cancer can be used as important reference index for prognosis assessment, but not as independent predictors of prognosis index.