摘要
目的探讨T细胞受体保守域α链基因(TCRCα-575A/G)多态性与过敏性紫癜(HSP)发病的关系。方法应用聚合酶链反应.限制内切酶片段长度多态分析(PCR—PFLP)方法结合琼脂糖凝胶电泳技术,对36例过敏性紫癜患儿和60例正常对照组儿童进行TCRCα-575A/G基因多态性分析。结果紫癜组TCRCα基因-575位点的AA、AG、GG基因型频率(22.2%、47.2%、30.6%)与正常组(28.3%、50.0%、21.7%)比较差异无统计学意义(P〉0.05),该位点的等位基因频率A、G(45.8%,54.2%)与正常组(53.3%、46.7%)比较差异无统计学意义(P〉0.05)。结论TCRCα-575A/G基因多态性变化与HSP的发生无明显关联性。
Objective To investigate the genetic polymorphisms of the T cell receptor constant al- pha chain gene (TCRCα) loci -575 A/G in Hunan Han Chinese children with Henoch-Schonlein purpura (HSP). Methods Thirty-six cases of patients with HSP and 60 healthy children as controls were investi- gated in this study. The T cell receptor constant alpha chain gene (TCRCα)loci - 575 A/G was detected with polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP). Results The genotype frequencies of AA, AG, and GG in the locus -575 A/G of TCRCα gene were 22. 2%, 47. 2% , and 30. 6% in HSP patients, and 28.3%, 50. 0%, and 21.7% in control group; and no signifi- cant differences were found between HSP patient and control groups ( P 〉 0. 05 ). The allele frequencies of A and G were 45.8% and 54. 2% in HSP patients ,and 53.3% and 46. 7% in control group; and no signif- icant differences were found between HSP patient and control groups ( P 〉 0. 05). Conclusions The pol- ymorphism of locus -575 A/G of T cell receptor constant alpha chain gene had not been found association with HSP.
出处
《中国医师杂志》
CAS
2013年第4期469-471,共3页
Journal of Chinese Physician
关键词
基因
T细胞受体
多态性
单核苷酸
紫癜
过敏性
汉族
儿童
湖南
Genes, T-cell receptor
Polymorphism, single nucleotide
Purpura, schoenlein-he-noch
HAN NATIONALITY
Child
HUNAN