长沙汉族儿童T细胞受体保守域α链基因-575A／G多态性与过敏性紫癜发病的关系

Study on the relationship of the T cell receptor constant alpha chain gene （TCRCα） loci -575 A/Gpolymorphisms and Henoch-Schonlein purpura （HSP） in Changsha Chinese Children

目的探讨T细胞受体保守域α链基因（TCRCα-575A／G）多态性与过敏性紫癜（HSP）发病的关系。方法应用聚合酶链反应．限制内切酶片段长度多态分析（PCR—PFLP）方法结合琼脂糖凝胶电泳技术，对36例过敏性紫癜患儿和60例正常对照组儿童进行TCRCα-575A／G基因多态性分析。结果紫癜组TCRCα基因-575位点的AA、AG、GG基因型频率（22．2％、47．2％、30．6％）与正常组（28．3％、50．0％、21．7％）比较差异无统计学意义（P〉0．05），该位点的等位基因频率A、G（45．8％，54．2％）与正常组（53．3％、46．7％）比较差异无统计学意义（P〉0．05）。结论TCRCα-575A／G基因多态性变化与HSP的发生无明显关联性。

Objective To investigate the genetic polymorphisms of the T cell receptor constant al- pha chain gene （TCRCα） loci -575 A/G in Hunan Han Chinese children with Henoch-Schonlein purpura （HSP）. Methods Thirty-six cases of patients with HSP and 60 healthy children as controls were investi- gated in this study. The T cell receptor constant alpha chain gene （TCRCα）loci - 575 A/G was detected with polymerase chain reaction-restriction fragment length polymorphism method （PCR-RFLP）. Results The genotype frequencies of AA, AG, and GG in the locus -575 A/G of TCRCα gene were 22. 2%, 47. 2% , and 30. 6% in HSP patients, and 28.3%, 50. 0%, and 21.7% in control group; and no signifi- cant differences were found between HSP patient and control groups （ P 〉 0. 05 ）. The allele frequencies of A and G were 45.8% and 54. 2% in HSP patients ,and 53.3% and 46. 7% in control group; and no signif- icant differences were found between HSP patient and control groups （ P 〉 0. 05）. Conclusions The pol- ymorphism of locus -575 A/G of T cell receptor constant alpha chain gene had not been found association with HSP.