负载转化生长因子β3微球的壳聚糖三维支架的制备

Preparation of controlled-released three-dimensional chitosan scaffold loading with TGF-β3 microspheres

目的探讨制备负载转化生长因子β3微球的壳聚糖三维支架的可行性。方法利用冻干法制备壳聚糖三维支架,扫描电镜观察并测定其水结合率及孔隙率。利用乳化交联法制备负载转化生长因子β3的壳聚糖微球,检测负载微球的体外缓释情况及吸水膨胀率。制作负载转化生长因子β3微球的壳聚糖三维支架,扫描电镜观察支架形态特征。结果壳聚糖三维支架孔隙较为一致,孔隙直径(180.4±35.3)μm,孔隙率(83.2±0.6)%,与水的结合能力为(123±5)%。电镜观察微球表面光滑,分散性好,粒径(28.5±5.1)μm。对壳聚糖微球体外缓慢释放TGF-β3连续监测7 d,总释放率为(46.2±0.3)%。负载TGF1-β3微球的壳聚糖三维支架观察见微球在支架中分布均匀。结论负载TGF-β3微球的壳聚糖三维支架的制备技术成熟,理化性质稳定,微球缓释TGF-β3效果理想,可作为理想的组织工程材料。

Objective To prepare a three-dimensional chitosan scaffold loading with transforming growth factor-β3 （TGF-β3） microspheres. Methods Three-dimensional chitosan scaffold was prepared by lyophilization, and water bonding capacity and porosity were measured. TGF-β3 loaded chitosan microspheres were prepared by emulsion-crosslinking method. The controlled release of TGF-β3 was monitored for 7 d and the swelling index was measured. At last, the three-dimensional chitosan scaffold loading with TGF-β3 microspheres was prepared and observed by scanning electron microscope （SEM）. Results SEM images showed the morphology of porous scaffold was uniform, and the mean diameter of interconnected pores was 180.4±35.3 μm. The porosity was （83.2±0.6）% and swelling index of microspheres was （123±5）%. The microspheres were spherical and smooth, and the mean diameter of the microspheres was 28.5±5.1 μm. The accumulated release of TGF-β3 reached （46.2±0.3）% within 7 d. Most microspheres were uniform in the scaffold. Conclusion The method for preparing three-dimensional chitosan scaffold loading with TGF-β3 microsphere is feasible, and the sustained release of TGF-β3 is realized.