Snail在卵巢癌细胞侵袭转移潜能方面研究

Role of Snail in the Invasion and Migration of Ovarian Carcinoma Cells

目的研究Snail与卵巢癌侵袭转移问的关系，并探讨其分子机制。方法分别构建正义全长Snail真核表达载体和小分子干扰RNA，分别转染卵巢癌细胞系A2780、C13＊。采用Western印迹方法分析相关蛋白表达，Transwell试验检测细胞侵袭转移能力。结果①成功构建Snail正义全长真核表达载体，并合成Snail小分子RNA干扰片段；②在转染PEGFPCI／Snail的卵巢癌细胞株A2780、C13＊中E-eadhefin表达明显下调，Snail和Vimentin则表达上调；而在转染Snail／SiRNA的卵巢癌细胞株A2780、C13‘中E—cadherin表达明显上调，Snail和Vimentin则表达下调；③Snail过表达可显著促进卵巢癌细胞株A2780、C13＊的侵袭转移潜能；封闭Snail表达水平可一定水平抑制卵巢癌细胞株A2780、C13＊的侵袭转移潜能。结论snail可通过促进卵巢癌上皮细胞间质化转变（epithelial—mesenchymaltransition，EMT）而提高其侵袭转移能力；封闭Snail表达可一定程度逆转卵巢癌上皮细胞间质化转变（EMT）而抑制其侵袭转移能力。

Objective To examine the relationship between Snail and the invasion and migra- tion of ovarian carcinoma cells and the underlying molecular mechanism. Methods The eukaryotic expression vector carrying full-length sense Snail gene and the Snail-targeting small molecule RNA interference fragment were constructed and transfected into A2780 and C13 ＊ cells （two ovarian car- cinoma cell lines） respectively. Western blotting was used to detect the expression of Snail, E-cad- herin and Vimentin. The invasive and migratory ability of cells was examined by transwell as- say. Results The Snail over-expressing plasmid PEGFPC1/Snail and the small molecule RNA in- terference fragment Snail/siRNA were successfully constructed. After the PEGFPC1/Snail transfec- tion, the expression level of E-cadherin was significantly decreased and that of Snail and Vimentin substantially increased in A2780 and C13 ＊ cells. Transfection with Snail/SiRNA resulted in an in-crease in the expression of E-cadherin and a decrease in the expression of Snail and Vimentin in A2780 and C13 ＊ cells. Snail overexpression contributed to the invasion and migration of A2780 and C13 ＊ cells and Snail silencing inhibited such abilities of the cells. Conclusion Snail may enhance the potential of invasion and migration of ovarian carcinoma cells by inducing epithelial-mesenchymal transition （EMT） . Snail silencing may inhibit the cell invasion and migration by reversing the change of EMT.