应用细胞损伤和反应指标评估体外氧化应激模型

Evaluation of Oxidative Stress Models by Cellular Damage and Reaction

目的应用细胞损伤及反应评估两种氧化应激模型在相关疾病研究中的应用。方法分别采用高糖和氧化剂Rosup培养HepG2肝细胞，检测细胞内活性氧量、DNA损伤及DNA修复酶PARP的表达。结果高糖培养4d可诱导细胞内活性氧生成增加至对照组3倍，Rosup组增加至对照组2倍。DNA损伤状况，高糖组是对照组2．7倍，Rosup组是对照组3倍。细胞反应指标PARP活性，高糖组是对照组2．8倍，Rosup组是对照组4．4倍。结论高糖诱导细胞的损伤表现为逐渐累积的过程，更适用于模拟体内长期复杂的损伤状态：而化学氧化剂是一个快速、强烈的诱导过程，更适用于研究急性氧化损伤过程。

Objective To evaluate the application of two oxidative stress models in the study of related diseases by cellular damage and reaction. Methods HepG2 cells were cultured in media with high glucose concentration or oxidant Rosup. ROS generation, DNA damage, and the expres- sion of DNA repair enzyme PARP were detected. Results Both 30ram glucose and Rosup induced more accumulation of ROS （3-fold and 2-fold） than control. DNA damage was markedly strong in both high glucose-treated cells （2. 7-fold） and Rosup-treated cells （3-fold） . High glucose-treated cells showed higher PARP activity （2. 8-fold） than control. Rosup-treated cells increased PARP ac- tivity 4.4-fold compared to control group. Conclusions High glucose concentration exhibited a gen- tly accumulation process of cellular damage and eellular reaction, which was close to glucose toxici- ty in pathophysiology. However, chemical oxidant induced a fast and strong effect, which was suit- able for the research on acute oxidative damage.