非缺氧条件下血府逐瘀汤促内皮细胞血管形成中bFGF作用的实验研究

Roles of bFGF in Endothelial Cell Tube Formation Induced by Xuefu Zhuyu Decoction under Non-anoxia Condition

目的探讨在非缺氧条件下血府逐瘀汤对碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)促血管新生中的作用。方法运用血清药理学方法,在常规95%O2培养条件下,以1.25%、2.50%、5.00%浓度的血府逐瘀汤含药血清和空白血清干预血管内皮细胞ECV30448h,通过体外成血管实验、酶联免疫法和逆转录PCR法分别检测不同浓度血府逐瘀汤含药血清对管腔形成、bFGF的含量和转录水平的影响。结果 1.25%、2.50%、5.00%含药血清均可明显促进内皮细胞参与管腔形成,尤以1.25%、2.50%含药血清诱导形成的管腔形态规整,而且能显著提高细胞培养上清液中bFGF的含量和其转录水平。结论在非缺氧条件下血府逐瘀汤通过上调bFGF的表达,发挥促血管新生作用。

Objective To explore the roles of basic fibroblast growth factor （bFGF） on tube formation induced by Xuefu Zhuyu Decoction （XZD） under non-anoxia condition. Methods Using serum pharmacology technique, endothelial cell line ECV304 cells were incubated in routine 95% O2. ECV304 cells were intervened by 1.25%, 2.50%, and 5.00% XZD containing serums and the vehicle serum for 48 h. The effects of XZD on tube formation, bFGF contents and its transcription levels were assessed by in vitro tube formation assay, enzyme-linked immunosorbent assay （ELISA）, and reverse transcriptase polymerase chain reaction （RT-PCR）, respectively. Results Three concentrations of XZD containing serums could not only obviously promote the tube formation bFGF level, but also up-regulate bFGF contents in the supernate and its transcription levels. The shapes of lumens were more regular in those induced by 1.25% and 2.50% XZD containing serums. Conclusion XZD induced angiogenesis via up-regulating the bFGF expression under non-anoxia condition.