血府逐瘀口服液干预应激抑郁模型大鼠的实验研究

Xuefu Zhuyu Oral Liquid Intervened Stress-stimulated Depression Model Rats

目的观察血府逐瘀口服液对抑郁模型大鼠大脑行为学和5-羟色胺(5-HT)及脑源性神经营养因子(BDNF)含量的影响。方法将雄性SD大鼠随机分为对照组、模型组、血府逐瘀组、黛力新组,每组12只。以慢性轻度不可预见性的应激法建立抑郁大鼠模型,血府逐瘀组和黛力新组大鼠分别给予血府逐瘀口服液、黛力新片灌胃干预21天。采用旷野实验和蔗糖水消耗实验观察大鼠行为学改变,并检测5-HT和BDNF含量。结果实验大鼠经过慢性应激性刺激后,可出现行为异常改变,模型组5-HT含量降低,与对照组比较,差异有统计学意义(P<0.01)。经血府逐瘀口服液和黛力新片干预后,应激大鼠行为学未发现明显改变,5-HT含量降低不明显(P>0.05),且血府逐瘀组5-HT含量高于黛力新组(P<0.05)。但模型组大鼠BDNF含量与对照组比较,差异无统计学意义(P>0.05),而血府逐瘀组和黛力新组大鼠BDNF含量低于模型组(P<0.01)。结论应激可以导致大鼠行为学改变和5-HT含量降低而出现抑郁倾向。血府逐瘀口服液干预可对抗抑郁引起的行为学改变,增加5-HT含量;但对大鼠BDNF含量影响并不明显,考虑其可能不通过BDNF途径起效。

Objective To observe effects of Xuefu Zhuyu Oral Liquid （XZOL） on the brain behavior and monoamine neurotransmitter 5-HT, and brain derived neurotrophic factor （BDNF） content on depression model rats. Methods Male SD rats were randomly divided into the control group, the model group, the XZOL group, and the Deanxit Tablet group, 12 in each group. The depressive rat model was established by chronic unpredictable mild stress method. XZOL was administered to rats in the XZOL group by gastrogavage, while Deanxit Tablet was given to those in the Deanxit Tablet group by gastrogavage. The intervention lasted for two weeks. The behavioral changes were observed by sucrose water consumption test and open-field test. The 5-HT and BDNF contents were detected using ELISA. Results After chronic stress stimulus, experimental rats in the model group might have abnormal behavioral changes and lowered 5-HT content, showing statistical difference when compared with the control group （P 0.01）. No obvious change in stimulated rats′ behavior after intervention of XZOL and Deanxit Tablet. 5-HT content was not obviously reduced （P 0.05）. Besides, XZOL was superior to Deanxit Tablet in increasing the 5-HT content （P 0.05）. But the brain BDNF level of rats in the model group was not statistically different from that of rats in the model group （P 0.05）, while the brain BDNF level of rats in the XZOL group and the Deanxit Tablet group was lower than that of rats in the model group （P 0.01）. Conclusions Stress can lead to behavioral changes and lowered 5-HT content of rats. The intervention of XZOL could fight against depression-induced behavioral changes and increase 5-HT content. But it did not significantly affect the brain BDNF level. We inferred that it might not effect through the BDNF pathway.