摘要
目的构建大容量且能高效展示于哺乳动物细胞表面的全长人源膀胱癌特异性抗体基因库。方法分离膀胱癌患者的外周血单核细胞(PBMC),提取PBMC的总RNA,用RT-PCR方法扩增全套IgG1重链可变区(VH)和Kappa型轻链全长(LCκ)基因,经过相应的限制性内切酶酶切后分别插入pDGB-HC-TM载体,分别构建膀胱癌特异性的抗体重链基因库和轻链基因库(一级抗体库)。随机挑取20个单克隆测定抗体基因序列,并瞬时转染FCHO细胞,结合流式细胞仪检测细胞表面抗体的表达。将上述构建好的轻重链基因库通过4片段连接方法插入双表达载体pDGB4,构建二级抗体库,转染FCHO后用流式细胞仪检测细胞表面抗体表达。结果分别成功构建了膀胱癌特异性的重链基因库和轻链基因库,随机挑取的轻重链单克隆各有7个和9个含有正确的抗体基因编码序列,且能展示于哺乳动物细胞表面,理论库容量达到3.32×10^(11)[(1.7×10~6×70%)×(3.1×10~5×90%)]。成功构建了膀胱癌二级抗体库,库容量为9×10~5。结论利用哺乳动物细胞表面展示技术,我们成功构建了膀胱癌特异性的全长人源抗体基因库,一级抗体库的库容量达到3.32×10^(11),二级抗体库库容量达到9×10~5,为下一步筛选特异性、高亲和力抗膀胱癌抗体奠定了基础。
Objective To construct full-length human bladder cancer-specific antibody libraries for efficient display of full-length antibodies on the surface of mammalian cells. Methods The total RNA was isolated from peripheral blood mononuclear cells from patients with bladder cancer. The repertoires of IgG1 heavy chain variable region (VII) and Kappa light chain were amplified by RT-PCR using specific primers. The antibody genes were inserted into the vector pDGB-HC-TM to construct the bladder-cancer-specific antibody libraries of heavy chains and light chains. Ten clones from each library were randomly picked for gene sequencing and transient transfection into FCHO cells to analyze antibody display on mammalian cell surface by flow cytometry after staining with corresponding fluorescent labeled antibodies. Results The libraries of bladder-cancer-specific antibody heavy chain (IgG1) and light chain (LOc) were successfully constructed. Seven out of the 10 clones randomly selected from the heavy chain library and 9 out of the 10 clones from the light chain library showed correct open reading frame, coding for 7 unique VH and 9 unique LCk. The combinatory library size reached 3.32×1011[(1.7×106×70%) × (3.1 × 105x 90?/o)]. Conclusion We have successfully constructed a full-length human bladder-cancer-specific antibody library with a combinatory diversity of 3.32 × 1011 based on mammalian display technology which can be used for screening monoclonal antibodies antinst bladder-cancer-associated antigens.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2013年第5期684-691,共8页
Journal of Southern Medical University
基金
广东省自然科学基金(S2011010003912,S2012040006972)
教育部高等学校博士学科点专项科研基金(20124433120002)
杭州环捷科技有限公司科研基金
广东省教育厅育苗工程项目(LYM11037)
南方医科大学科研启动计划