Helokinestatin对大鼠平滑肌的毒性研究

Toxicological effects of helokinestatin on smooth muscle cells in rats

目的:研究毒蜥毒素中Helokinestatin的4种亚型对大鼠尾动脉平滑肌的毒性作用。方法:取成年雄性大鼠尾动脉,分离得到平滑肌肌束。将动脉平滑肌标本连到张力换能器上,分别以不同浓度的bradykinin(缓激肽)和bradykinin、Helokinestatin联合作用于大鼠平滑肌,统计各个浓度的肌肉收缩张力的相对值作为纵坐标,以多肽浓度的-log值作为横坐标进行线性回归作图,进行统计学分析。观察4种Helokinestatin处理标本的肌张力改变情况。结果:与单纯给予bradykinin处理相比,同时给予bradykinin和Helokinestatin处理的标本,在多肽浓度为10^(-7)M^10^(-5)M的肌张力明显增加,差异有统计学意义(P<0.05)。结论:.4种Helokinestatin在多肽浓度为10^(-7)M^10^(-5)M时,都能拮抗缓激肽对大鼠平滑肌的舒张作用.并且这种拮抗作用是非竞争性的。

Objective：To study the toxicological effects of four subtypes of Helokinestatins derived from Heledermatidae lizards on tail artery smooth muscle cells in rats. Methods：Smooth muscle bundles derived from the tail artery in male adult rats were adhered to a tension transducer. This was followed by administration of different concentrations of bradykinin or the combination with Helokinestatin. The linear regression figure, as depicted by plotting the relative values of muscle tension at different concentrations of bradykinin against the negative logarithms of the concentration of polypeptide, was employed for statistical analysis on the changes in muscle tone stimulated by different concentrations of stimuli. Results： The addition of Helokinestatin, compared to bradykinin alone, yielded an increase in the muscle tone within the polypepfide concentration of 10-7mol/L to 10-5 mol/L（ P 〈 0.05 ）. Conclusion： The four subtypes of Halokinastatin may non-competitively antagonize the relaxation effects induced by bradykinin on rat tail artery smooth muscle at the polypeptide concentration of between 10-7mol/L and 10-5 mol/L.