慢性HCV感染者外周血自然杀伤细胞亚群的检测及临床意义

Determination of peripheral blood natural killer cell subsets in patients with chronic HCV infection and analysis of its clinical significance

目的检测慢性HCV感染者外周血自然杀伤细胞(natural killer cells,NK细胞)不同亚群的比例,并分析临床意义。方法采用流式细胞仪技术,检测慢性HCV感染者外周血NK细胞亚群的比例,并观察抗病毒治疗前后NK细胞亚群的变化及与抗病毒疗效的关系。结果与对照组比较,慢性HCV感染组外周血NK细胞中NKdim亚群比例低,NKnegative亚群高(P均<0.05);抗病毒治疗后NKbright亚群比例上升,NKdim亚群下降(P均<0.05);抗病毒治疗有效组NKbright亚群比例低于无效组,NKdim亚群比例高于无效组(P均<0.05)。结论慢性HCV感染者NK细胞亚群较健康人群有差异,且与抗病毒疗效相关。

Objective To determine the frequencies of different subsets of peripheral blood natural killer （NK） cells in patients with chronic HCV infection, and to investigate the NK subset changes and its clinical significance. Methods Flow cytometry was used to determine the frequencies of different subsets of peripheral blood NK ceils in patients with chronic HCV infection, and the corre- lation between the changes in NK cell subsets before and after antiviral therapy and antiviral efficacy was analyzed. Results Compared to the healthy controls, the frequency of NK subset was lower （P〈0.05）, and that of NK subset higher in chronic HCV patients （P〈0.05）. After antiviral treatment, the frequency of NKb subset increased （P〈0.05）, and that of NK subset decreased （P〈0.05）. The patients with early virological response exhibited a lower frequency of NKsht subset and a higher frequency of NKm subset than those with no antiviral response （P〈0.05）. Conclusions Peripheral blood NK cell subsets in patients with chronic HCV infection differ from those of healthy controls and are correlated with antiviral efficacy.