摘要
目的观察tocilizumab对肺动脉高压大鼠白细胞介素-6(IL-6)和survivin的表达水平的影响。方法通过腹腔注射野百合碱诱导建立肺动脉高压大鼠模型,观察tocilizumab对肺动脉高压大鼠IL-6和survivin的表达水平的影响。结果 Tocilizumab干预组大鼠和空白对照组大鼠mPAP、IL-6和survivin含量明显低于实验对照组,P<0.05。Tocilizumab干预组肺动脉血管内膜轻度水肿,血管壁轻度增厚。IL-6和survivin在实验对照组大鼠肺组织和血清中表达强烈,在tocilizumab干预组大鼠肺组织少量表达,而空白对照组大鼠肺组织不表达。结论 Tocilizumab可有效抑制野百合碱可诱导肺动脉高压大鼠IL-6和survivin的表达,抑制肺动脉高压的发生。
Objective To observe the effects of tocilizumab on IL 6 and survivin expression in pulmonary arterial hypertension rat model. Methods The pulmonary arterial hypertension rat model induced by monocrotaline. IL-6 and survivin expression on pulmonary vascular and serum were observed. Results MPAP of rats in both tocilizumab inter vention group and blank control group significantly lower than experimental control group, P〈~0. 05. HE staining showed that mild intimal edema and mild thickening vessel of pulmonary artery wall in tocilizumab intervention group. Immunohistochemistry showed expression of II. 6 and survivin obviously on the lung tissue in the experimental control, a small amount of expression in the tocilizumab intervention group. Conclusion Tocilizumab could effectively inhibit expression of IL-6 and survivin and development of pulmonary hypertension induced by monocrotaline.
出处
《中国实验诊断学》
2013年第5期820-822,共3页
Chinese Journal of Laboratory Diagnosis
