摘要
植物叶绿体基因组DNA(cpDNA)的非编码序列是研究植物分子系统发育、群体遗传学和建立植物DNA条形码(Barcodes)的重要工具.为了筛选适宜的cpDNA片段研究柳穿鱼(Linaria vulgaris)遗传多样性,本研究对柳穿鱼4个自然群体中叶绿体基因组的4个DNA非编码片段rpl32-trnL、trnQ-5rps16、trnT-trnL、trnS-trnG进行测序研究,序列比对和系统发育分析显示不同的cpDNA片段具有不同的分子变异水平.其中,rpl32-trnL的比对长度为805bp,蕴含24个潜在的简约性信息位点,且在柳穿鱼种内群体间的遗传分化明显.本研究筛选到rpl32-trnL作为下一步研究柳穿鱼群体遗传学和生物地理学的理想分子标记.
Non coding fragments of chloroplast DNA（cpDNA） are important tools in study of plant molecular phylogenetics,population genetics and construction of plant DNA barcodes.In order to choose applicable cpDNA regions to investigate population genetic diversity of Linaria,four cpDNA fragments,rpl32-trnL,trnQ-5＇rps16,trnT-trnL,trnS-trnG in four natural populations of Linaria vulgaris are analyzed.The results of sequences alignment and phylogenetic analysis imply that there are various variations in different regions of cpDNA.The rpl32-trnL is 805 bp in long after aligning,which contains 24 potential parsimony information sites and differentiates apparently among populations of this species in preliminary molecular systematic analysis.It is regarded as ideal molecular marker for study of population genetics and biogeography in next step.
出处
《西北师范大学学报（自然科学版）》
CAS
北大核心
2013年第3期71-75,91,共6页
Journal of Northwest Normal University(Natural Science)
基金
国家自然科学基金资助项目(31060033)
