植物抗冻蛋白分离纯化方法的研究进展

Research Progress in Separation and Purification Methods of Antifreeze Proteins from Plants

为植物抗冻蛋白是一类具有热滞活性的蛋白质,它能抑制冰晶的生长和重结晶。因其特殊的结构和功能,植物抗冻蛋白对延长冷冻食品的贮藏期和提高产品质量具有积极意义。从复杂提取液中高效分离纯化出抗冻蛋白已成为当前热点和难点问题,作者介绍了近年来植物抗冻蛋白分离纯化方法的基本原理和研究进展,主要有传统层析分离方法、SDS-PAGE电泳、冰特异吸附分离法和浊点萃取法,展望了抗冻蛋白分离纯化方法的未来发展方向。

Antifreeze proteins are the thermal hysteresis proteins ,which have the ability to modify ice growth and inhibit ice recrystallization. Antifreeze proteins from plants are of significance to elongate shelf-life of frozen food and improve their quality. How to separate antifreeze proteins from complicated extract liquids effectively has been a hot and challenging topic. In this article,the recent advances in the separation and purification methods of antifreeze proteins from plants are reviewed and the basic principles of the methods are introduced. The methods include traditional chromatography separation,SDS-PAGE eleetrophoresis,iee-affinity adsorption and cloud point extraction. Based on the above research progress, the future directions to develop separation methods for antifreeze proteins are put forward.